Growth Factor Reduced (GFR) formulation is well suited for applications that require a more highly defined basement membrane preparation.

• Mouse colonies routinely screened for pathogens via Mouse Antibody Production (MAP) testing
• Extensive PCR testing is performed to screen for a number of pathogens
• Tested and found negative for bacteria, fungi, and mycoplasma
• Protein concentrations determined by Lowry method
• Endotoxin units measured by Limulus Amoebocyte Lysate assay
• Matrigel Matrix gel stability tested for a period of 14 days at 37 °C
• Biological activity determined for each lot using a neurite outgrowth assay.

Corning Matrigel matrix is a solubilised basement membrane preparation extracted from the Engelbreth-Holm-Swarm (EHS) mouse sarcoma, a tumor rich in extracellular matrix proteins, including Laminin (a major component), Collagen IV, heparin sulfate proteoglycans, entactin/nidogen, and a number of growth factors.

Application
• Cell Growth and Differentiation
• Metabolism/Toxicology Studies
• Invasion Assays
• In Vitro and In Vivo Angiogenesis Assays
• In Vivo Angiogenesis Studies and Augmentation of Tumors in Immunosuppressed Mice

Preparation and Storage
Store at –20 °C. Avoid multiple freeze-thaws.

Note: For research use only. Not intended for diagnostic or therapeutic procedures.

When warmed, the solution polymerizes to produce a biologically active matrix material resembling the mammalian cellular basement membrane. It is ideal for use with in vitro cell cultures of a broad range of cell types, including both primary and stem cell applications.