For digestion of native elastin.

• Digest native elastin effectively
• Use in combination with other proteinases for successful digestion of fibrous tissue

Digest native elastin in cell cultures with Elastase. This serine protease is frequently used in combination with other proteases such as collagenase and trypsin to digest fibrous tissue. Elastase consists of a single polypeptide chain of 240 amino acid residues and contains four disulfide bridges (Shotton and Hartley). It is synthesized as a zymogen (proelastase) and then converted to the active form by limited trypsin proteolysis at its N-terminal. Elastase preferentially cleaves the peptide bond of C-terminal neutral, non-aromatic amino acid residues (Schellenberger et al.).