Mitochondrial Isolation Kit

Isolation kit to enrich the mitochondrial subcellular fraction from mammalian cells or tissue - 50 tests.

• Fast and efficient enrichment of the active mitochondrial subcellular fraction
• Verified for use with western blot analysis

Conveniently enrich the mitochondrial or cytosolic subcellular fractions from mammalian cells or tissues using the Mitochondrial Isolation Kit. This kit offers a quick mitochondrial isolation technique that has been verified for detection using western blot analysis and may be used to study apoptosis and signaling events between the two fractions.

The Mitochondrial Isolation Kit offers two options to isolate the mitochondrial subcellular fraction. The first option is a reagent-based method for processing up to six samples concurrently. The second method utilizes Dounce homogenization, providing a two-fold increase in isolated mitochondria from a single sample. Both methods employ microcentrifugation to separate the mitochondrial fraction from the cytosolic fraction.

The enriched mitochondrial fractions preserve their biological activity and are compatible with downstream applications, including the study of mitochondrial respiration, apoptosis, inner mitochondrial membrane potential, and protein profiling (Itahana and Zhang Cancer Cell, 2008).

Achtung! Bitte beachten: Product restrictions: (i) Customer shall not make products or any portion of them in any way, shape or form, including as a component of another product available for the purpose of further resale or alter or remove the Product label and the Supplier’s mark of origin without express written permission of Supplier and (ii) Customer is familiar with Supplier’s restrictions on the use of the products and shall use the products in compliance with such restrictions and the applicable Supplier Product Information sheet (collectively, the 'Product Restrictions').