Human Aldose Reductase ELISA kit is a 90 minute sandwich Enzyme-Linked Immunosorbent Assay (sELISA) designed for the in vitro quantitative determination of human Aldose Reductase in serum, plasma, and other biological fluids.

• Higher throughput: Get results in just 90 minutes, with a single wash step
• Assay precision: Intra-assay: CV <8%, Inter-assay: CV <10%

Human Aldose Reductase ELISA kit employs the sandwich enzyme immunoassay technique for the quantitative measurement of human Aldose Reductase in serum, plasma or other biological fluids. An antibody specific for Aldose Reductase has been pre-coated onto a 96-well microtiter plate. The standards and test samples are added into the wells and the Aldose Reductase present in each sample is bound to the wells by the immobilised antibody. Biotinylated anti-Aldose Reductase antibody, which also binds the Aldose Reductase present in each sample, and Streptavidin-HRP, which binds the Biotinylated anti-Aldose Reductase antibody, are added and the microtiter plate is incubated. Following incubation, unbound Biotinylated anti-Aldose Reductase antibody and unbound Streptavidin-HRP are removed by washing, and two substrate solutions are added to the wells. Colour develops in proportion to the amount of Aldose Reductase captured in each well. The colour development is stopped by addition of stop solution which changes the colour from blue to yellow and the intensity of the colour is then measured. The concentration of Aldose Reductase in the samples can then be calculated by reading the O.D. absorbance at 450 nm in a microplate reader and referring to the standard curve.