Human Clathrin Light Chain ELISA Kit

Human Clathrin Light Chain ELISA kit is a 90 minute sandwich Enzyme-Linked Immunosorbent Assay (sELISA) designed for the in vitro quantitative determination of human Clathrin Light Chain in serum, plasma, and other biological fluids.

• Higher throughput: Get results in just 90 minutes, with a single wash step
• Assay precision: Intra-assay: CV <8%, Inter-assay: CV <10%

Human Clathrin Light Chain ELISA kit employs the sandwich enzyme immunoassay technique for the quantitative measurement of human Clathrin Light Chain in serum, plasma or other biological fluids. An antibody specific for Clathrin Light Chain has been pre-coated onto a 96-well microtiter plate. The standards and test samples are added into the wells and the Clathrin Light Chain present in each sample is bound to the wells by the immobilised antibody. Biotinylated anti-Clathrin Light Chain antibody, which also binds the Clathrin Light Chain present in each sample, and Streptavidin-HRP, which binds the Biotinylated anti-Clathrin Light Chain antibody, are added and the microtiter plate is incubated. Following incubation, unbound Biotinylated anti-Clathrin Light Chain antibody and unbound Streptavidin-HRP are removed by washing, and two substrate solutions are added to the wells. Colour develops in proportion to the amount of Clathrin Light Chain captured in each well. The colour development is stopped by addition of stop solution which changes the colour from blue to yellow and the intensity of the colour is then measured. The concentration of Clathrin Light Chain in the samples can then be calculated by reading the O.D. absorbance at 450 nm in a microplate reader and referring to the standard curve.

Bestellinformation: This is a ready-to-use ELISA kit that contains: Pre-coated 96 well microplate, positive control, negative control, HRP-conjugated detection antibody, sample diluent, substrate solution A, substrate solution B, stop solution, wash buffer (25X), plate sealers, foil pouch, and protocol manual.