- VE:1 KIT
- Bezeichnung:STEMdiff™ NK cell kit
For expansion and differentiation of hPSCs to NK cells.
Differentiate human pluripotent stem cells (hPSCs) to NK cells with the feeder-free and serum-free STEMdiff™ NK Cell Kit. The STEMdiff™ NK Cell Kit protocol first uses animal component-free STEMdiff™ Hematopoietic - EB reagents for the generation of embryoid bodies (EBs) from hPSCs which are then further differentiated into CD34+ cells.
The kit includes all the EB reagents necessary for this step:
STEMdiff™ Hematopoietic - EB Basal Medium
STEMdiff™ Hematopoietic - EB Supplement A
STEMdiff™ Hematopoietic - EB Supplement B
The CD34+ cells are then further directed to differentiate to CD56+ NK cells using StemSpan™ reagents:
StemSpan™ SFEM II
StemSpan™ Lymphoid Progenitor Expansion Supplement (10X)
StemSpan™ Lymphoid Differentiation Coating Material (100X)
StemSpan™ NK Cell Differentiation Supplement (100X)
For convenience, the STEMdiff™ Hematopoietic - EB and StemSpan™ reagents included with the kit are also available individually.