Cell culture medium kit for robust generation of midbrain neural organoids from human pluripotent stem cells.

Robustly generate three-dimensional, patterned brain organoid cultures from human pluripotent stem cells without matrix embedding by using STEMdiff™ midbrain organoid differentiation kit. When paired with AggreWell™800™, this serum-free cell culture media can prevent organoid fusion and support the generation of over 500 organoids per kit for higher-powered statistical replicates and more detailed longitudinal study. Adapted from protocols, the brain-region-specific organoids generated with this kit are three-dimensional in vitro models with a cellular composition and structural organization representative of the developing human midbrain.

They are consistently sized with reproducible morphology between various cell lines, and provide a reliable model for the study of dopaminergic neurons within a functional 3D system. Midbrain organoids can be combined with striatal organoids generated using the STEMdiff™ Dorsal Forebrain Organoid Differentiation Kit with additional patterning factors to generate midbrain-striatal AssemBloid™ cultures. Organoids can be maintained with STEMdiff™ neural organoid maintenance kit to support long-term culture survival (>50 days) for predictive assays, high-throughput phenotypic screening, and neurotoxicity assays.