Serum- and BPE-free medium for expansion of primary human keratinocytes.

Improve derivation efficiency and expansion rates for long-term culture of proliferative human primary keratinocytes using BPE- and serum-free DermaCult™ keratinocyte expansion medium and a feeder-free protocol. More efficient cell expansion means that you can perform repeats and technical replicates from the same sample and avoid purchasing additional undifferentiated cells or sourcing patient-derived samples. Unlike other commercial expansion media, DermaCult™ keratinocyte expansion medium enables you to obtain >50 population doublings of human neonatal keratinocytes and >35 population doublings of human adult keratinocytes. With the flexibility to change the media every two days and cryopreserve proliferative cells at any passage - while maintaining their differentiation potential - you can carry out your research at your convenience.

Cells expanded in this medium exhibit cobblestone morphology and express key basal cell markers (K14, p63, ITAG6, and ITGB1) representative of basal keratinocytes and are suitable for differentiation to air-liquid interface (ALI) cultures. Formulated without serum or BPE to maximise experimental reproducibility, confidently use keratinocytes cultured in DermaCult™ for in vitro models to study skin and inflammatory diseases, perform irritation and corrosion assays, or to assess the efficacy of cosmetics or drugs. DermaCult™ keratinocyte expansion medium is compatible for passaging with ACCUTASE™ at 3 to 5 day intervals. It is also suitable for use with collagen, such as to establish keratinocyte culture from skin tissue.