For the quantification of LTE₄ in urine.

• Measure LTE₄ in urine, with no purification required
• Assay 24 samples in triplicate or 36 samples in duplicate
• Measure LTE₄ levels down to 25 pg/ml
• Incubation: 18 hours, Development: 90 to 120 minutes, Read: Colorimetric at 405 to 420 nm

Leukotriene E4 (LTE₄) is a product of the 5-lipoxygenase (5-LO) pathway in activated mast cells, eosinophils, and monocytes. LTA₄, the primary 5-LO metabolite, is rapidly converted to LTC₄ and sequentially to LTD₄ and LTE₄. While plasma levels of LTC₄ are virtually undetectable, exogenously administered LTC₄ is recovered in the urine as LTE₄ (5 to 13%) and two prominent oxidized metabolites resulting from several cycles of β-oxidation. Cayman’s LTE₄ ELISA Kit is a competitive assay developed for the measurement of LTE₄ from urine without a purification step. The assay has a range of 7,8 to 1000 pg/ml and a sensitivity (80% B/B₀) of approximately 25 pg/ml. This ‘dilute and go’ format has been used successfully to demonstrate significant and reproducible changes in LTE₄ levels in the urine of asthmatic patients after antigen challenge. If your samples require additional purification prior to analysis, Cayman’s Cysteinyl Leukotriene Affinity Sorbent can be used for this purpose.

Contents: Antiserum, Tracer, Standard, Buffer, Polysorbate 20, Reagent, Dyes, Plate, and Cover Sheet