Spezifikationen

• Konjugation:
  Unconjugated
• Protein/Peptid-Typ:
  Rekombinant
• Ursprung:
  HEK293 cells
• Spezies:
  Human
• Tag sequence:
  Fc Tag
• Lagerbedingungen:
  –20 °C
• Endotoxingehalt:
  Less than 1,0 EU per μg by the LAL method.
• Biologische Aktivität:
  Measured by its binding ability in a functional ELISA. Immobilised Human IL-12B, His Tag (Cat. No. NK2-H52H7) at 5 μg/ml (100 μl/well) can bind Human IL-12 R beta 1, Fc Tag (Cat. No. ILB-H5255) with a linear range of 5 - 78 ng/ml (QC tested). Human IL-12 R beta 1, Fc Tag (Cat. No. ILB-H5255) captured on CM5 chip via Anti-human IgG Fc antibodies surface can bind Human IL-12B&IL-12A Heterodimer Protein, His Tag&Flag Tag (Cat. No. IL2-H4210) with an affinity constant of 2,06 nM as determined in a SPR assay (Biacore T200) (Routinely tested). Loaded Human IL-12 R beta 1, Fc Tag (Cat. No. ILB-H5255) on Protein A Biosensor, can bind Human IL-12B, His Tag (Cat. No. NK2-H52H7) with an affinity constant of 7,14 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested). Loaded Human IL-12B&IL-12A Heterodimer Protein, His Tag&Flag Tag (Cat. No. IL2-H4210) on HIS1K Biosensor, can bind Human IL-12 R beta 1, Fc Tag (Cat. No. ILB-H5255) with an affinity constant of 32,3 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
• Gen-ID:
  P42701-1
• Proteinsynonym:
  CD212|IL12RB|IL-12 R beta 1|IL12RB1|IL-12RB1|IL12R
• Protein/Peptid-Name:
  IL-12 R beta 1
• Purity:
  >95% as determined by reduced SDS-PAGE.
• Molecular weight:
  83,5 kDa
• Endotoxinspiegel:
  Low
• Zusammensetzung:
  Lyophilised from 0,22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH 7,5. Normally trehalose is added as protectant before lyophilisation.
• Transporttemperatur:
  RT