Anti-Tnfrsf16 Mouse Monoclonal Antibody [clone: MC192] Saporin

MC192-saporin is an antibody conjugate comprising of the monoclonal antibody MC192 against rat p75NTR, the nerve growth factor receptor, chemically conjugated via a reducible disulfide bridge to the ribosome-inactivating protein saporin, purified from saponaria officinalis.

Specific only for rat NGFR
No reactivity to human or mouse NGFR has been reported
Does not cross react with p75NTR-expressing cells in other species than rat

Unconjugated saporin is incapable of entering the cells due to the apparent lack of ligand. Upon specific binding via MC192 to the cells expressing p75NTR, saporin transverses the cell membrane leading to lesion of neurochemically defined neuronal populations. The targets of MC192-Saporin are p75NTR-expressing cells including cholinergic neurons of the basal forebrain, cerebellar Purkinje cells, medial septum, diagonal band of broca, nucleus basalis of Meynert and some tumor cells. MC192-saporin has been used in the study of learning and memory and its primary application is in vivo, MC192-saporin is specific for applications in rat. The antibody does not cross-react with human or mouse p75NTR receptors.

Other application: A model for gene delivery into neurons.

: Only qualified personnel should handle this product. MC192 antibody does not bind to human p75NTR receptor, however, standard laboratory protective measures should be followed, including wearing lab coat, gloves and safety glasses. Avoid contact with open wounds and practice good laboratory technique to avoid auto-exposure/auto-injection! Avoid contact of conjugate with reducing agents (eg., DTT, beta-mercaptoethanol) as this will destroy the antibody-saporin conjugate.

Type: Primary
Antigen: NGFR
Clonality: Monoclonal
Clone: MC192
Conjugation: Saporin
Epitope: Extracellular
Host: Mouse
Isotype: IgG1
Reactivity: Rat

Catalog No:
BSENM-1755-50-IT
BSENM-1755-100-IT
BSENM-1755-25-IT

Antigen-Symbol:
Tnfrsf16
Tnfrsf16
Tnfrsf16

Antigen-Name:
NGFR/p75 neurotrophin receptor (p75NTR)
NGFR/p75 neurotrophin receptor (p75NTR)
NGFR/p75 neurotrophin receptor (p75NTR)

Antigen-Synonyme:
Low affinity neurotrophin receptor p75NTR|CD271|NGF receptor|p75 ICD|Gp80-LNGFR|Low-affinity nerve growth factor receptor
Low affinity neurotrophin receptor p75NTR|CD271|NGF receptor|p75 ICD|Gp80-LNGFR|Low-affinity nerve growth factor receptor
Low affinity neurotrophin receptor p75NTR|CD271|NGF receptor|p75 ICD|Gp80-LNGFR|Low-affinity nerve growth factor receptor

Antikörper-Typ:
Primär
Primär
Primär

Klonalität:
Monoklonal
Monoklonal
Monoklonal

Konjugation:
Saporin
Saporin
Saporin

Klon:
MC192
MC192
MC192

Reaktivität:
Rat
Rat
Rat

Wirt:
Mouse
Mouse
Mouse

Gen-ID:
UniprotID #P07174, P20656
UniprotID #P07174, P20656
UniprotID #P07174, P20656

Isotyp:
IgG1
IgG1
IgG1

Epitop:
Extracellular
Extracellular
Extracellular

Immunogen:
Rat NGF receptor (p75NTR)
Rat NGF receptor (p75NTR)
Rat NGF receptor (p75NTR)

Aminosäurezahl:
299 amino acids
299 amino acids
299 amino acids

Form:
Lyophillised
Lyophillised
Lyophillised

Molecular weight:
30 kDa
30 kDa
30 kDa

Reinigung:
Conjugate was purified by ion-exchange chromatography. Purity >90% by non-reducing SDS-PAGE
Conjugate was purified by ion-exchange chromatography. Purity >90% by non-reducing SDS-PAGE
Conjugate was purified by ion-exchange chromatography. Purity >90% by non-reducing SDS-PAGE

Konzentration:
1 mg/ml
1 mg/ml
1 mg/ml

Lagerungspuffer:
Reconstitute in sterile-filtered, ultrapure water to achieve a conjugate concentration of 1 mg/ml. Centrifuge to remove any insoluble material. Final buffer contains no preservatives.
Reconstitute in sterile-filtered, ultrapure water to achieve a conjugate concentration of 1 mg/ml. Centrifuge to remove any insoluble material. Final buffer contains no preservatives.
Reconstitute in sterile-filtered, ultrapure water to achieve a conjugate concentration of 1 mg/ml. Centrifuge to remove any insoluble material. Final buffer contains no preservatives.