Creatinine colorimetric/fluorometric assay kit

Creatinine assay kit provides an accurate, convenient measure of creatine concentration in biological fluids such as serum, urine or CSF.

• Reactivity: Mammals
• Detection Method: Colorimetric, fluorometric

In the assay, creatinine is converted to creatine by creatininase, creatine is converted to sarcosine, which is specifically oxidised to produce a product which reacts with a probe to generate red colour (OD 570 nm) and fluorescence (Ex/Em = 538/587 nm). Unlike the picric acid assay, this kit is suitable for serum/plasma creatinine determinations, as well as for urine and other biological samples.

Suitable Sample: Biological Fluid, Cell Culture Supernatant, Plasma, Serum, Tissue Culture Supernatant, Urine.

Delivery information: Kit includes creatinine assay buffer (25 ml), creatinine probe (0,2 ml), creatinase (1 vial), creatininase (lyophilised,1 vial), creatinine enzyme mix (lyophilised, 1 vial) and creatinine (10 µmol, lyophilised (1 vial).

Caution: Regulation Status:For research use only (RUO). Storage Instruction:Store the kit at -20°C