Rat Recombinant TNFalpha (from E. coli)

Tumor necrosis factor alpha (TNF-α) is an inflammatory cytokine secreted by macrophages, monocytes, neutrophils, T cells, and NK-cells following stimulation by bacterial lipopolysaccharide (LPS). TNF-α signal activation occurs through two receptors, TNFR1 and TNFR2. TNFR1 is expressed on most cell types, unlike TNFR2, which is expressed mainly on immune cells. TNF-α functions to stimulate phagocytosis in macrophages, chemoattract neutrophils, increase insulin resistance, and induce fever.

High Quality
Low Endotoxin
Affordable

A COA is supplied with each product shipped detailing purity, formulation, biological activity, endotoxin levels, reconstitution instructions and recommended storage conditions. This product is for research use only and is not for use in humans.

: For research use only.

Konjugation:
Unconjugated
Protein-Funktion:
Cytokine
Protein/Peptid-Typ:
Rekombinant
Ursprung:
E. coli
Spezies:
Rat
Lagerbedingungen:
–20 °C
Endotoxingehalt:
Endotoxin LAL (EU/µg) ≤1
Biologische Aktivität:
Bioactivity assay: Cytolysis of mouse L929 cells in the presence of Actinomycin D, ED50 ≤0,05 ng/ml, Bioactivity ≥2,0×10⁷ units/mg
Gen-ID:
P16599
Reconstitution instructions:
Sterile water at 0.1 mg/ml
Frei von Endotoxinen:
N
Frei von Trägern:
Y
Frei von Proteasen:
N
Tierfrei:
N
Proteinsynonym:
Cytotoxin|DIF|Necrosin|Cachexin|TNFSF2|TNF|Cachectin
Protein/Peptid-Name:
TNFalpha
Purity:
≥95%
Molecular weight:
17,3 kDa
Sequence:
MLRSSSQNSS DKPVAHVVAN HQAEEQLEWL SQRANALLAN GMDLKDNQLV VPADGLYLIY SQVLFKGQGC PDYVLLTHTV SRFATSYQEK VSLLSAIKSP CPKDTPEGAE LKPWYEPMYL GGVSQLEKGD LLSAEVNLPK YLDITESGQV YFGVIAL
Endotoxinspiegel:
Low
Zusammensetzung:
10 mM sodium phosphate, 50 mM sodium chloride, pH 7,5
Frei von Nukleasen:
N
Sorte:
RUO
Transporttemperatur:
RT
Validierte Anwendungen:
Bioassay