Human recombinant GALE (from E. coli)
PRSI91-243
:
- VE:50 µG
- Protein/Peptid-Typ:Rekombinant
- Ursprung:E. coli
- Spezies:Human
- Tag sequence:N-6 His tag
- Lagerbedingungen:Store at −20 °C, stable for 6 months after receipt. Please minimize freeze-thaw cycles.
- Proteinsynonym:Galactowaldenase|GALE|UDP-Glucose 4-Epimerase|UDP-Galactose 4-Epimerase
- Protein/Peptid-Name:GALE
- Purity:> 95% as determined by reducing SDS-PAGE
- Molecular weight:40.44 kD
- Sequence:Met1-Ala348
- Zusammensetzung:Supplied as a 0.2 um filtered solution of 50mM TrisHCl, 150mM NaCl, 2mM DTT, 1mM EDTA, pH 8.0. Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 ug/ml. Dissolve the lyophilized protein in ddH2O. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
- Validierte Anwendungen:Bioassay
The enzyme UDP-Glucose 4-Epimerase (GALE) is a homodimeric epimerase found in bacterial, plant and mammalian cells. UDP-Glucose 4-Epimerase performs the final step in the Leloir pathway of Galactose metabolism, it catalyzes two distinct but analogous reactions: the epimerization of UDP-Gglucose to UDP-Galactose and the epimerization of UDP-N-Acetylglucosamine to UDP-N-Acetylgalactosamine. The bifunctional nature of the enzyme has the important metabolic consequence that mutant cells (or individuals) are dependent not only on exogenous galactose, but also on exogenous N-acetylgalactosamine as a necessary precursor for the synthesis of glycoproteins and glycolipids.
- This recombinant protein can be used for biological assays. For research use only.
Fusion-Tag: N-6 His tag