Specifications
- VE:50 µG
- Protein/Peptid-Typ:Rekombinant
- Ursprung:E. coli
- Spezies:Human
- Lagerbedingungen:Lyophilized protein should be stored at −20 °C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 4-7 °C for 2-7 days. Aliquots of reconstituted samples are stable at −20 °C for 3 months.
- Biologische Aktivität:ED50 is 0.5 ng/ml as determined by the dose-dependent inhibition of IL-1 stimulation of D10S cells.
Specific Activity of 2.0 x 106 IU/mg. - Proteinsynonym:IL1F3|IL-1ra|IL-1RN|Interleukin-1 Receptor Antagonist Protein|IL-1F3|IL1 Inhibitor|IRAP|IL1RN|ICIL-1RA|Anakinra|IL1RA
- Protein/Peptid-Name:IL1RA
- Purity:> 95% as determined by reducing SDS-PAGE
- Molecular weight:17.26 kD
- Sequence:Arg26-Glu177
- Zusammensetzung:Lyophilized from a 0.2 um filtered solution of 50mM TrisHCl, 200mM NaCl, pH 7.5. Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 ug/ml. Dissolve the Lyophilized protein in ddH2O. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
- Validierte Anwendungen:Bioassay
Specifications
About this item
Interleukin-1 Receptor Antagonist (IL-1RN) is a member of the IL-1 family. Endogenous IL-1RN is produced in numerous animal disease models as well as in human autoimmune and chronic inflammatory diseases. It binds to IL-1 receptors in competition with IL-1, but does not elicit intracellular response from this binding. Its role in counteracting the proinflammatory effects of IL-1 is being studied by numerous research groups. IL-4 and IL-13 have been shown to amplify the stimulatory effect of IL1-beta on the production of soluble and intracellular forms of IL-1RN. The regulated expression of IL-1RN in various cell types has been shown to be influenced by cytokines. In synovial fibroblasts, IL-1, TNF-alpha, or PDGF markedly enhances the synthesis of IL-1RN.
- This recombinant protein can be used for biological assays. For research use only.