The 1-Step™ Ultra TMB-ELISA contains soluble TMB (3,3´,5,5´-tetramentylbenzidine) that is ideal for sensitive ELISA-based applications. TMB is a chromogenic substrate for horseradish peroxidase. Once oxidised by the enzyme, this substrate yields a blue product that absorbs at 370 nm and 652 nm. Adding sulfuric or phosphoric acid to stop the reaction changes the product to yellow that absorbs at 450 nm and provides greater sensitivity than the blue product. The 1-Step Ultra TMB-ELISA is supplied ready to use with no mixing required.

• No additional reagents or filtering required
• Non carcinogenic
• No DMF or DMSO present

Substrate type: HRP (Horseradish Peroxidase)
Detection method: Colorimeteric method