Affinity chromatography columns, HiTrap™ IMAC HP

HiTrap IMAC HP is prepacked with IMAC Sepharose High Performance. The resin is charged with the metalion of your choice for Immobilized Metal ion Affinity Chromatography (IMAC) and subsequent high-resolution purification of polyhistidine tagged proteins.

• For optimizing purification of histidine-tagged proteins when Ni2+ is not the best choice of metal ion
• Conveniently charge with your metal of choice
• Small bead size for high-performance purification
• High binding capacity

IMAC Sepharose HP is supplied free of metal ions. It is charged by the user with the transition metal ion of choice (e.g. Cu2+, Zn2+, Ni2+, or Co2+); these metal ions will bind to the covalently immobilized chelating ligand on the Sepharose.