Ihre Suche nach: Nukleinsäure-Reagenzien

Supplier: AAT BIOQUEST

Real-time thermal cyclers require calibration to generate a spectral profile for the fluorescence signal.

Supplier: FINNZYMES REAGENTS

Phusion® Blood Direct PCR Master Mix is designed for amplification of DNA from whole blood. It eliminates the need for a separate DNA purification step prior to PCR. The modified Phusion® Hot Start II High-Fidelity DNA Polymerase is resistant to main PCR inhibitors present in blood and retains its activity at blood concentrations of up to 40% in the PCR reaction.

Supplier: FINNZYMES REAGENTS

Phire® Hot Start II DNA Polymerase is faster, extremely robust, and capable of amplifying long DNA fragments with high yields. Phire® Hot Start II DNA Polymerase incorporates a dsDNA-binding domain which allows short extension times (10 to 15 s/kb), improves yields, and increases fidelity 2-fold compared to Taq DNA polymerase. In addition, the Hot Start technology allows complete reactivation of the enzyme in “zero-time” at standard cycling temperatures. This combination of features makes the polymerase an ideal solution for routine and high throughput PCR applications. Phire® Hot Start II DNA Polymerase delivers superior performance in conventional thermal cyclers as well as in fast instruments, such as the Piko® Thermal Cycler.

Supplier: Cytiva

Ready-To-Go™ You-Prime First-Strand beads are preformulated, single-dose reaction beads prepackaged in thin-walled 0,5 ml tubes compatible with most thermal cyclers. Ready-To-Go™ You-Prime First-Strand beads are used for synthesis of first-strand cDNA templates from total RNA or polyadenylated RNA using a primer of choice.

Supplier: AAT BIOQUEST

ROX dye qPCR Calibration Solution supplied as a 10000X concentrate is compatible with a wide variety of qPCR instruments, including the ABI7500 Fast, QuantStudio™, and ViiA™ 7 systems.

Supplier: Thermo Fisher Scientific

Luminaris Color Probe qPCR Master Mixes are universal ready-to-use solutions optimised for qPCR and two-step RT-qPCR. The master mixes include hot start Taq DNA polymerase, uracil-DNA glycosylase (UDG) and dNTPs in an optimised PCR buffer. Only the template, primers and probe need to be added.

Supplier: Thermo Fisher Scientific

The Maxima™ Probe qPCR Master Mix (2X) is a ready to use solution optimised for quantitative real time PCR and two-step real time RT-PCR on most real time PCR machines. The master mix includes Maxima™ hot start Taq DNA polymerase and dNTPs in an optimised PCR buffer. dUTP is included in the mix for optional carryover contamination control using uracil-DNA glycosylase (UDG). The use of Maxima™ probe qPCR master mix (2X) in real time PCR ensures reproducible, sensitive and specific quantification of genomic, plasmid, viral and cDNA templates.

Supplier: FINNZYMES REAGENTS

Direct PCR Kits save time and cost by allowing amplification of DNA directly from the source material. Direct PCR is based on Thermo Scientific Phire® Hot Start II DNA Polymerase and Phusion® Hot Start II High-Fidelity DNA Polymerase. These fusion enzymes are exceptionally fast, robust and highly tolerant of many PCR inhibitors present in unpurified sample material. This allows reliable amplification in challenging conditions where conventional DNA polymerases are completely inhibited.

Supplier: Thermo Fisher Scientific

PCR Master Mix is a 2X concentrated solution of Taq DNA polymerase, dNTPs and all other components required for PCR, except DNA template and primers. This pre-mixed formulation saves time and reduces contamination due to a reduced number of pipetting steps required for PCR set up. The mix is optimised for efficient and reproducible PCR.

Supplier: Merck Millipore (Novagen)

Premixed 2X KOD Hot Start PCR components for convenience and reproducibility.

Supplier: FINNZYMES REAGENTS

Phire® Hot Start II DNA Polymerase is faster, extremely robust, and capable of amplifying long DNA fragments with high yields. Phire® Hot Start II DNA Polymerase incorporates a dsDNA-binding domain which allows short extension times (10 to 15 s/kb), improves yields, and increases fidelity 2-fold compared to Taq DNA polymerase. In addition, the Hot Start technology allows complete reactivation of the enzyme in “zero-time” at standard cycling temperatures. This combination of features makes the polymerase an ideal solution for routine and high throughput PCR applications. Phire® Hot Start II DNA Polymerase delivers superior performance in conventional thermal cyclers as well as in fast instruments, such as the Piko® Thermal Cycler.

Supplier: MP Biomedicals

Hybrisol VII

Supplier: Cytiva

Designed to generate full length first-strand cDNA from mRNA templates. Following first-strand cDNA synthesis, the sample can be used directly for in vitro amplification using PCR or for second-strand synthesis using the Gubler-Hoffman method.

Supplier: VIAGEN BIOTECH

Schnelles und kostengünstiges 'Single-Tube-System' zur schnellen Präparation von PCR-Template DNA aus Mausschwänzen und -ohren, embryonalem Gewebe und Säugerzellen.

Supplier: G-Biosciences

Taq DNA Polymerase is a highly thermostable recombinant DNA polymerase derived from the thermophile Thermus aquaticus. The molecular weight of the recombinant protein is 94 kD. The Taq Polymerase is able to amplify DNA up to 5 kb with an elongation velocity of 0,9 to 1,2 kb/min at 70 to 75 °C. The error rate of this Taq Polymerase is ~2,2×10⁻⁵ nucleotide⁻¹ cycle⁻¹. Taq DNA polymerase catalyses the 5’→3’ synthesis of DNA. The enzyme has no detectable 3’→5’ proofreading exonuclease activity, and possesses low 5’→3’ exonuclease activity, which results in a 3’-dA overhang on the PCR product

Supplier: MP Biomedicals

Taq-&GO™ is a ready to use optimised reaction mix for PCR.

Supplier: Cytiva

T4 DNA ligase catalyses the formation of a phosphodiester bond between the 5'-phosphoryl group and the 3'-hydroxyl group of two double-stranded DNA fragments. ATP is required for this reaction.

Supplier: MP Biomedicals

The Taq CORE kits are designed for the amplification of specific regions of DNA using PCR. The PCR CORE Kits - Direct Loading provide Taq DNA Polymerase, PCR Direct Loading buffers and dNTPs necessary for amplification and subsequent loading of samples on an agarose gel.

Supplier: MP Biomedicals

Taq-&LOAD™ PCR Master Mix is an optimised 5X reaction mix for PCR containing recombinant Taq DNA polymerase, dNTPs, MgCl₂, and incubation and loading buffers. Also included are a compound increasing the density of the sample and a red/purple dye.

Supplier: MP Biomedicals

Taq DNA Polymerase is a specialised thermostable enzyme isolated from the thermophilic bacterium Thermus aquaticus. The recombinant form of this enzyme is expressed in E. coli. It is highly processive, recombinant polymerase which catalyses the addition of mononucleotide units to the 3’-OH end of a primer chain. It remains functional even after prolonged incubation at 95 °C. This enzyme possesses 5’-3’ exonuclease activiy.

Supplier: G-Biosciences

Pfu DNA Polymerase, derived from the hyperthermophilic archae Pyrococcus furiosus, has superior thermostability and proofreading properties compared to other thermostable polymerases. It has a molecular weight of 90 kDa and can amplify DNA targets up to 2 kb. The elongation velocity is 0,2~0,4 kb/min (70~75 °C). Pfu DNA Polymerase possesses 3' to 5' exonuclease proofreading activity that enables the polymerase to correct nucleotide misincorporation errors. This means that Pfu DNA Polymerase-generated PCR fragments will have fewer errors than Taq-generated PCR inserts. Using Pfu DNA Polymerase results in blunt-ended PCR products, which are ideal for cloning into blunt-ended vectors. Pfu DNA Polymerase is superior for techniques that require high-fidelity DNA synthesis.

Supplier: Merck Millipore (Novagen)

Die KOD HiFi DNA-Polymerase ist ein einzigartiges Proofreading-Enzym, das aus dem extrem thermophilen Thermococcus kodakaraensis KOD1 isoliert wird (mit höherer Aktivität und Genauigkeit) und somit eine schnellere und genauere PCR-Amplifizierung ermöglicht, als sie mit herkömmlichen Enzymen erreicht werden kann.

Supplier: Biotium

A qPCR master mix containing Evagreen® qPCR dye and Cheetah™ Taq hotstart DNA polymerase, suitable for qPCR using a fast cycling protocol.

Supplier: Biotium

EvaGreen® Plus Dye has an improved signal-to-noise compared to original EvaGreen® dye, for higher sensitivity in DNA amplification applications.

Supplier: Merck Millipore (Novagen)

Der NovaTaq PCR MasterMix ist eine gebrauchsfertige, 2-fach konzentrierte Mischung aus NovaTaq DNS-Polymerase, ultrareinen Desoxynukleotiden und einem Reaktionspuffer ohne Magnesiumchlorid. Der MasterMix erleichtert die Vorbereitung von PCR Reaktionen und bietet die folgenden Vorteile: Zeitersparnis, Konsistenz und minimales Kontaminationsrisiko. Fügen Sie einfach den NovaTaq PCR MasterMix zu einem gleichen Volumen mit den erforderlichen Mengen an Magnesiumchlorid, DNS-Template und Primern hinzu, und die Reaktion ist zum Thermocycling bereit. Die fertig verdünnte Reaktion enthält 2,5 Einheiten NovaTaq DNS-Polymerase pro 100 µl. In der Lieferung sind genug Komponenten für 200 standardmäßige 50-µl-Amplifizierungsreaktionen (bzw. 100×100 µl) enthalten.

Supplier: Merck Millipore (Novagen)

KOD Xtreme™ Hot Start DNA Polymerase is an ultra high fidelity DNA polymerase designed for the most challenging PCR applications, including crude sample PCR, amplification of long strand and highly GC-rich DNA templates.

Supplier: STEMCELL Technologies

Immunmagnetische Extraktion der gesamten Nukleinsäure (DNA und RNA).

Supplier: MACHEREY-NAGEL

NucleoSpin® RNA mini spin kit for the isolation of RNA of the highest integrity.

Supplier: MACHEREY-NAGEL

NucleoSpin® Plant II Midi/Maxi-Kits sind für die Isolierung genomischer DNA aus Pflanzenmaterial und Pilzen konzipiert. Plant II Midi ermöglicht die Verarbeitung von bis zu 400 mg Ausgangsmaterial und Plant II Maxi bis zu 1500 mg.

Supplier: MACHEREY-NAGEL

NucleoSpin® 8/96 Tissue und NucleoSpin® 8/96 Tissue Core Kits zur Isolierung genomischer DNA aus menschlichem oder tierischem Gewebe, kultivierten Zellen oder Bakterien. Erhältlich mit einer Auswahl an flexiblem 8-Well-Streifenformat für unterschiedlichen Durchsatz oder 96-Well-Format für hohen Durchsatz.