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Ihre Suche nach: Nukleinsäure-Reagenzien

Nukleinsäure-Reagenzien

Nukleinsäure-Reagenzien bilden die Grundlage der genetischen Analyse und Molekularbiologie und spielen eine zentrale Rolle von der Klonierung bis hin zu Genexpressionsstudien. Tauchen Sie ein in unsere Sammlung von hochpräzisen Enzymen für Endpunkt-PCR, fortschrittlichen Reagenzien für isotherme Amplifikation und umfassenden Kits zur Nukleinsäureaufreinigung. Für Präzision in quantitativen Assays entdecken Sie unsere Auswahl an qPCR- und RT-qPCR-Kits, die für unvergleichliche Genauigkeit optimiert sind. Unser Portfolio umfasst auch Reagenzien der nächsten Generation für das genomische Sequenzieren und Fortschritte in der personalisierten Medizin.

DNA polymerase, hotstart, PfuUltra II Fusion

Supplier: AGILENT

This DNA polymerase is ideal for a variety of techniques requiring high-fidelity DNA synthesis by the polymerase chain reaction (PCR).

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Pfu DNA polymerase

Supplier: AGILENT

High-quality polymerases for difficult to amplify DNA targets.

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Restriction enzymes, rTaqPDNA

Supplier: Apollo Scientific

Recombinant Taq plus DNA polymerase produced in E.coli 5 U/µl.

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PerfeCTa® qPCR FastMix™ Reaktionsmischungen

Supplier: Quantabio

Die FastMixes enthalten Puffer und Stabilisatoren, die für maximale Effizienz, Reproduzierbarkeit und Empfindlichkeit der PCR mit TaqMan® und TaqMan® MGB-Sondenchemie optimiert sind. Ein Hauptbestandteil der PerfeCTa® qPCR FastMixes ist die AccuFast™ Taq DNA-Polymerase, die eine sofortige Aktivierung und schnelle Polymerisationskinetik ermöglicht. Nach der Wärmeaktivierung (20 Sekunden bei 95 °C) denaturieren die Antikörper irreversibel und setzen vollständig aktive, unveränderte Taq DNA-Polymerase frei. So ist eine spezifische und effiziente Primer-Extension mit dem Vorteil des Reaktionsaufbaus bei Zimmertemperatur möglich. FastMixes liefern maximale PCR-Effizienz, -Empfindlichkeit, -Spezifität und ein starkes Fluoreszenzsignal mit entweder schnellen oder herkömmlichen Cycling-Bedingungen.

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AccuStart™ II PCR Genotyping Kit

AccuStart™ II PCR Genotyping Kit

Supplier: Quantabio

The AccuStart™ II Mouse Genotyping kit is designed for fast and easy preparation of PCR-ready DNA extracts and endpoint PCR analysis from tissues such as tail snips and ear punches commonly used for genotyping knockout and transgenic animals. The kit combines Extracta™ DNA Prep for PCR - Tissue with AccuStart™ II GelTrack™ PCR SuperMix in a single kit.

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PerfeCTa® SYBR® Green SuperMixes und FastMixes™

PerfeCTa® SYBR® Green SuperMixes und FastMixes™

Supplier: Quantabio

PerfeCTa® SYBR® Green SuperMixes und FastMixes™ ermöglichen effiziente, empfindliche und präzise quantitative PCR mit proprietären Puffern und SYBR® Green Stabilisatoren, die das Fluoreszenzsignal und die PCR-Effizienz maximieren und Primerdimeren verringern. Die SuperMixes und FastMixes™ wurden für alle Echtzeit-PCR-Instrumentenplattformen optimiert, einschließlich der Plattformen, bei denen eine Normalisierung mit ROX-Referenzfarbstoff oder Fluorescin erforderlich ist.

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PerfeCTa® qPCR FastMix® II

Supplier: Quantabio

PerfeCTa® qPCR FastMix® II is an advanced qPCR reagent system for both fast and conventional PCR cycling protocols or instruments. It is a versatile and robust solution that provides the ultimate sensitivity and high PCR efficiency using a variety of fluorogenic probe chemistries, including TaqMan® hydrolysis probes. PerfeCTa® qPCR FastMix® II is provided as a 2X concentrated ready to use reaction cocktail that contains all required reaction components, except primers, probe(s), and DNA template. The light blue colour of the AccuVue™ tracer dye simplifies reaction assembly in white, or clear, plates and helps to minimise pipetting or mixing errors. It does not interfere with qPCR performance or affect the stability of the product.

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Brilliant III Ultra-fast probe ROX qPCR master mix

Supplier: AGILENT

High performance, ultra-sensitive, probe QPCR master mix reagent with high or low ROX concentration for reliable quantification across a wide range of targets and templates.

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Paq5000 DNA polymerase

Supplier: AGILENT

Paq5000 is capable of quickly and robustly amplifying up to 6 kB genomic DNA targets.

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PicoMaxx high fidelity PCR system

Supplier: AGILENT

Detects low copy number target and withstands PCR inhibitors without extensive purification.

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Taq extender PCR additive

Supplier: AGILENT

Improves reliability and yield of conventional Taq-based PCR amplifications.

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One Step Fast qRT PCR Kits, qScript™

Supplier: Quantabio

Diese Kits bieten eine praktische und hochempfindliche Lösung für reverse Transkriptase-PCR (qRT-PCR) der RNA-Templates in Echtzeit unter Verwendung der Hybridisierungssonden für chemische Nachweisverfahren wie TaqMan® 5'-Hydrolyse-Proben.

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Viability PCR starter kits

Supplier: Biotium

Kits designed to make it simple to start performing viability PCR with the choice of viability dye (PMA or PMAxx™).

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AMPIGENE® qPCR 1-Step Probe Kit No-ROX

Supplier: ENZO LIFE SCIENCES

Optimised and efficient cDNA synthesis and probe-based qPCR in a single tube.

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AccuStart™ II Taq DNA polymerase

Supplier: Quantabio

AccuStart™ II Taq DNA Polymerase is a high purity, recombinant Taq DNA polymerase preparation with high avidity monoclonal antibodies that bind the polymerase and keep it inactive prior to the initial PCR denaturation step. Upon heat activation (1 minute at 94 °C), the antibodies denature irreversibly, releasing fully active, unmodified Taq DNA polymerase. This enables specific and efficient primer extension with the convenience of room temperature reaction assembly. Non-specific extension of primers at low temperatures is a common cause of artifacts and poor sensitivity in PCR. The AccuStart™ II automatic hot-start enables specific and efficient primer extension in the PCR process with the added convenience of room temperature reaction assembly. The included 10X PCR Buffer II is a new optimised buffer that provides higher product yield, improved specificity, and enhanced multiplexing capability.

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qPCR SuperMixes, PerfeCTa®

qPCR SuperMixes, PerfeCTa®

Supplier: Quantabio

PerfeCTa® qPCR SuperMixes sind 2X konzentrierte, gebrauchsfertige Reaktionscocktails, die alle Komponenten außer Primern, Sonde und Vorlage für qPCR enthalten.

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PfuUltra high-fidelity DNA polymerase

Supplier: AGILENT

PfuUltra High-Fidelity DNA Polymerase AD features a reformulated buffer system for increased economy with the same high performance as the original PfuUltra DNA Polymerase for robust, ultra-high-fidelity PCR.

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Taq DNA polymerase, Cheetah™ HotStart

Supplier: Biotium

Cheetah™ Taq is a chemically modified hot-start Taq DNA polymerase useful for preventing or reducing nonspecific DNA amplification in PCR. Modified using a novel modifying reagent, Cheetah™ Taq represents a major improvement upon AmpliTaq Gold by having a faster activation time and better shelf-life.

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BspQ I Restriction Endonuclease

Supplier: Abnova

The restriction endonuclease is a restriction endonuclease BspQ I that can recognize specific sites and is produced under GMP standards and expressed recombinantly in Escherichia coli. Restriction endonucleases are widely used in various fields such as gene positioning and cloning. This enzyme cleaves DNA rapidly for efficient gene linearization.

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dNTP set, MP Biomedicals

Supplier: MP Biomedicals

Set consists of 100 mM aqueous solutions of dATP, dCTP, dGTP and dTTP each supplied in a separate vial.

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illustra™ PCR nucleotide mix dNTP set

illustra™ PCR nucleotide mix dNTP set

Supplier: Cytiva

PCR nucleotide mix dNTP set (2 mM, 10 mM, or 25 mM each A,C,G,T) for amplification, dideoxy sequencing, labelling, mutagenesis, cDNA synthesis, and expression profiling.

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DNA isolation, tissue and cells genomicPrep mini spin kit, illustra™

DNA isolation, tissue and cells genomicPrep mini spin kit, illustra™

Supplier: Cytiva

illustra™ tissue and cells genomicPrep Mini Spin Kit is designed for the rapid extraction and purification of high quality genomic DNA from a variety of animal tissues and mammalian cell cultures. The kit can be used for several downstream applications including restriction enzyme analysis, ligation, cloning, DNA sequencing and PCR.

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illustra™ blood genomicPrep mini spin kit

illustra™ blood genomicPrep mini spin kit

Supplier: Cytiva

The blood genomicPrep Mini Spin Kit is designed for the rapid and reproducible isolation of high-quality genomic DNA from whole blood, buffy coat, bone marrow, and nucleated red blood cells.

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Nucleon™ BACC genomic DNA extraction kits

Nucleon™ BACC genomic DNA extraction kits

Supplier: Cytiva

Nucleon™ BACC Genomic DNA Extraction Kits were developed for rapid extraction of high quality, high molecular weight genomic DNA from blood and cell cultures.

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SPINeasy® DNA-Kit für Speichel

SPINeasy® DNA-Kit für Speichel

Supplier: MP Biomedicals

Das SPINeasy DNA-Kit für Speichel von MP Biomedicals erleichtert die Speichelprobenahme, indem es unsere speziell formulierte Speichel-Konservierungslösung (SPS) verwendet, um die Probe bei Raumtemperatur zu konservieren, ohne die Qualität zu beeinträchtigen.

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illustra™ triplePrep Kit

illustra™ triplePrep Kit

Supplier: Cytiva

Das illustra™ triplePrep Kit ist für die schnelle Isolierung und Aufreinigung mit hoher Ausbeute von genomischer DNA (gDNA), Gesamt-RNA, und denaturierten Gesamt-Proteinen aus ungeteiltem tierischen Gewebe und Säugerzellen konzipiert. Der optimierte Arbeitsablauf reduziert die Anzahl der Schritte, wodurch alle drei Analyten in weniger als 1 Stunde vorbereitet werden können.

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MicroSpin™ DNA Clean-up, Columns

MicroSpin™ DNA Clean-up, Columns

Supplier: Cytiva

MicroSpin™ columns are designed for the rapid purification of DNA in conjunction with Sephadex™ G-25 DNA grade for use in a wide range of applications, including desalting, buffer exchange and removal of unincorporated nucleotides from end-labelled oligonucleotides.

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RNA isolation, illustra™ RNAspin 96 Kit

RNA isolation, illustra™ RNAspin 96 Kit

Supplier: Cytiva

illustra™ RNAspin 96 kit is a flexible, high-throughput solution for total RNA isolation.

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Plasmid purification kit, Axygen® AxyPrep™ Mag

Plasmid purification kit, Axygen® AxyPrep™ Mag

Supplier: Corning

AxyPrep™ Mag Plasmid Purification Kit utilises a magnetic bead technology for high throughput purification of plasmid DNA from E.coli cells. The AxyPrep™ Mag kit can also be used with fosmid and BAC vector-based constructs ranging from 5 to 150 Kb. The system consists of steps of pelleting cells, resuspension, lysis, neutralisation, DNA binding, ethanol washing and plasmid elution. The protocol is automation-compatible.

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dNTP sets, illustra™

dNTP sets, illustra™

Supplier: Cytiva

High purity dNTPs for amplification, dideoxy sequencing, labelling, mutagenesis, cDNA synthesis, and expression profiling.

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