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Supplier: Enzo Life Sciences

The Adenovirus BioProbe® labeled probe is prepared by nick translation of cloned fragments of the adenovirus 5 genome. The fragments constitute a total of 20 kb of DNA, approximately 56% of the genome. The probe is specific for adenovirus and, in addition to hybridization to adenovirus 5 sequences, the probe will cross hybridize with adenovirus types 4, 7, 11, 20, 40 and 41 due to their considerable sequence homology. Fragment size range: 100-1000 base pairs (as estimated by agarose gel electrophoresis).

Supplier: Enzo Life Sciences

The BK Virus BioProbe® labeled probe is prepared by nick translation of a clone of the entire BK virus genome. BK virus, a simian polyoma virus, is related to SV40 and also bears some similarity to the human virus JC virus. BK virus is highly tumorigenic in hamsters. Fragment size range: 100-1000 base pairs (as estimated by agarose gel electrophoresis).

Supplier: Enzo Life Sciences

The Blur 8 BioProbe® labeled probe is prepared by nick translation of a clone of the human alu repeat sequence. Fragment size range: 100-1000 base pairs (as estimated by agarose gel electrophoresis).

Supplier: Thermo Fisher Scientific

For transformation of competent cells.

Supplier: BTX

Flatpack chambers are primarily used for prokaryotic applications (bacterial or yeast transformations), however they are also often used for high efficiency stem cell transfection.

Supplier: G-Biosciences

Fast Yeast Transformation™ is a rapid single step yeast transformation kit that takes less than 10 minutes to prepare competent yeast cells. The competent yeast cells can be used immediately or frozen for later use. This method is suitable for both circular and linear plasmid transformations.

Supplier: BTX

Die BTXpress™ ist eine einzelne Pufferlösung, die entwickelt wurde, um Gene schnell und effizient in Säugerzellen einzubringen, die bisher durch chemische und andere nicht-virale Verfahren als 'schwer transfizierbar' betrachtet wurden. Die Transfektion mit dieser hochleistungsfähigen Elektroporationslösung ist beim Einbringen von DNA sowie siRNA in Säugerzellen gleichermaßen effizient.

Supplier: Thermo Fisher Scientific

This T4 DNA ligase catalyzes the formation of a phosphodiester bond between juxtaposed 5'-phosphate and 3'-hydroxyl termini in duplex DNA or RNA. The enzyme repairs single-strand nicks in duplex DNA, RNA, or DNA/RNA hybrids. It also joins DNA fragments with either cohesive or blunt termini, but has no activity on single-stranded nucleic acids.

Supplier: BTX

BTXpress Cytoporation® Media T/T4 is an advanced electroporation buffer designed for use with the BTX AgilePulse MAX™ large volume electroporation system for in vitro delivery of DNA, RNA, oligonucleotides, and siRNA. The low conductivity buffer is specially formulated to minimise heating of solution during large volume electroporation for maximum transfection efficiency and high cell viability. BTXpress Cytoporation® Media T and T4 are sterile filtered from the highest quality non animal, medical grade reagents. Buffer can be directly diluted in complete growth media for post electroporation cell culture.

Supplier: AGILENT

Protein directed evolution in just one step.

Supplier: AGILENT

For libraries constructed from highly methylated DNA, Gigapack III Packaging Extract simplifies the packaging procedure and increases the efficiency and representation of libraries constructed from highly methylated DNA. Each packaging extract is restriction minus to optimize packaging efficiency and library representation.

Supplier: TriLink BioTechnologies

T7 RNA Polymerase is a wild-type enzyme to synthesize RNA from DNA templates by in vitro transcription.

Supplier: AGILENT

The GeneMorph II random mutagenesis kits have been formulated to produce a more uniform mutational spectrum when performing error prone PCR to achieve random mutagenesis.

Supplier: TriLink BioTechnologies

Inorganic Pyrophosphatase (iPPase) is an enzyme to remove pyrophosphate in IVT to drive the reaction forward.

Supplier: BTX

BTXpress Cytofusion® Medium C is an advanced electrofusion buffer designed especially for hybridoma production. The low conductivity buffer is specially formulated to minimise cell turbulence during cell alignment and heating during electrofusion, for robust cell fusion efficiency and high cell viability. BTXpress Cytofusion® Medium C is sterile filtered from the highest quality non animal, medical grade reagents.

Supplier: TriLink BioTechnologies

CleanScribe™ RNA Polymerase is a novel enzyme that reduces double-stranded RNA (dsRNA) formation in IVT RNA synthesis.

Supplier: TriLink BioTechnologies

TriLink's patented CleanCap® Reagent M6 [CleanCap m6AG (3′ OMe)], is designed for the co-transcriptional capping of mRNA to produce an mRNA with base-modified Cap 1. Cap-1 mRNAs have superior in vivo activity compared to Cap-0 mRNAs produced by legacy capping methods.

Supplier: Biotium

Digoxigenin-dUTP, alkali stable

Supplier: AGILENT

Pfu DNA ligase is a recombinant enzyme derived from Pyrococcus furiosus hyperthermophilic marine archaebacterium that catalyses linkage of adjacent 5'-phosphate and 3'-hydroxy ends of double-stranded DNA at 45 to 80 °C, with a temperature optimum near 70 °C for nick-sealing reactions.

Supplier: AGILENT

The StrataClone PCR cloning kit allows high-efficiency, 5-minute cloning of PCR products at room temperature, using the efficient DNA rejoining activity of DNA topoisomerase I and the DNA recombination activity of Cre recombinase.

Supplier: TriLink BioTechnologies

Engineered RNase Inhibitor is a protein to inhibit RNase activities to prevent RNA degradation in IVT.

Supplier: TriLink BioTechnologies

CleanCap® Reagent AU is designed for the co-transcriptional capping of self-replicating or self-amplifying (saRNA) to produce an optimal Cap 1.

Supplier: Thermo Fisher Scientific

T7 RNA polymerase is ideal for synthesis of unlabeled and labeled RNA. It catalyses the 5'→3' synthesis of RNA on either single-stranded DNA or double-stranded DNA downstream from it promoter. It is also used in the study of RNA secondary structure and RNA-protein interactions, RNA splicing.

Supplier: Thermo Fisher Scientific

The CloneJET PCR cloning kit contains a ready-to-use positive selection cloning vector pJET1.2/blunt. The vector contains a lethal restriction enzyme gene that is disrupted by ligation of a DNA insert into the cloning site. As a result, only bacterial cells with recombinant plasmids are able to form colonies. Recircularised pJET1.2/blunt vector molecules lacking an insert express a lethal restriction enzyme, which kills the host E. coli cell after transformation. This positive selection drastically accelerates the process of colony screening and eliminates additional costs required for blue/white selection.

Supplier: AGILENT

The original QuikChange Site-Directed Mutagenesis Kits speed up and simplify site-directed mutagenesis studies. The kits eliminate the need for sub-cloning into M13-based bacteriophage vectors and for ss-DNA rescue. This allows oligo-mediated introduction of site-specific mutations into virtually any double-stranded plasmid DNA. In addition, the XL version of the kit is specially designed for efficient mutagenesis of large (4 to 14 kb) or otherwise difficult-to mutagenise plasmid templates. The XL kit features components specifically designed for more efficient DNA replication and bacterial transformation.

Supplier: G-Biosciences

Plasmid Screening ToothPick™ allows the rapid screening of bacteria for transformed plasmids. Simply pick a colony, add to Plasmid Screening ToothPick™ reagents and then analyse using restriction enzymes.

Supplier: G-Biosciences

OmniPrep™ für grampositive Bakterien-Kit isoliert hochwertige genomische DNA aus grampositiven Bakterien. Bei protokollgemäßer Verwendung reinigt dieses Kit DNA aus 100 ml grampositiver Bakterienkultur.

Supplier: G-Biosciences

Das OmniPrep™-Kit isoliert hochwertige genomische DNA aus vielen verschiedenen Arten und Gewebetypen, darunter Tiere, Pflanzen, Bakterien, Hefen, Pilze, Vollblut und Zellen in Kultur. DNA kann aus Proben mit hohem Gehalt an Polysacchariden oder anderen Verunreinigungen isoliert werden, die sich nur schwer aus den DNA-Präparaten entfernen lassen.

Supplier: Sartorius

LentiSELECT kits offer LentiSELECT 40, LentiSELECT 500 and LentiSELECT 1000 for the purification and concentration of VSV-G pseudotyped lentivirus from 40 to 1000 ml cell culture, leading to purified infective particles. These lentivirus purification and concentration kits offer purified recombinant lentivirus particles for in-vitro transfection a fast, safe and easy to use solution. These kits replace time-consuming ultracentrifugation protocols, which typically take about one day for large sample volumes, thus reducing the purification time to only a few hours.

Supplier: G-Biosciences

GET™ CLEAN DNA uses high binding affinity GET™ spin columns to remove salts, enzymes, unincorporated nucleotides, radiolabels, and primer-dimers from any DNA preparation of 100 bp to 20 kb. GET™ spin columns has an enhanced binding affinity for DNA, thus eliminating loss or damage of DNA.