Ihre Suche nach: Protein Extraction Kits

Supplier: Abcam

Protein Extraction Kit (ab270054) is for the solubilization of proteins for a variety of proteomics applications.

Supplier: Abcam

Plasma membrane protein extraction kit provides optimized buffers and reagents for the effective extraction of plasma membrane proteins from mammalian tissues and cells. The kit offers the option to either extract total cellular membrane proteins or specifically purify plasma membrane proteins. - Cited in over 200 publications - High yield and purity – consistent yield with high purity (over 90%) - Suitable for western blotting, 2-D gels, enzyme analyses, and more

Supplier: G-Biosciences

Total Protein Extraction (TPE™) Kit is a universal lysis system for the solubilisation of total proteins from animal, plant, yeast, bacteria, and other biological samples. The kit contains ready to use buffers for extracting total protein from cells and tissues for SDS-PAGE analysis.

Supplier: Thermo Fisher Scientific

The Mem-PER™ Plus Membrane Protein Extraction Kit enables small-scale solubilisation and enrichment of integral membrane proteins and membrane-associated proteins in a simple reagent-based procedure. It effectively isolates membrane proteins from cultured cells and tissues using a simple benchtop microcentrifuge procedure.

Supplier: Thermo Fisher Scientific

B-PER® Bacterial Protein Extraction Reagents are designed to extract soluble protein from bacterial cells. The B-PER® Bacterial Protein Extraction Kit eliminates the need for harsh mechanical procedures, like sonication. The easy-to-use cell lysis solution uses mild non-ionic detergents to disrupt cells and solubilise proteins without denaturation. Enzyme supplements such as DNase I and Lysozyme allow for improved yield of large molecular weight proteins which are difficult to purify. B-PER® is also compatible with downstream protein purification methods such as GST purification and His-tagged immobilised metal affinity chromatography (IMAC).

Supplier: Merck Millipore (Calbiochem‎)

ProteoExtract® Kits cover the different steps of the proteomics workflow, from protein extraction and abundant protein removal to concentration of protein mixtures and removal of interfering substances, digestion of proteins and selective capturing of phosphopeptides. These kits work in concert with one another to provide protein samples that are directly compatible with downstream analytical applications.

Supplier: G-Biosciences

FOCUS™ Soluble & Insoluble is a complete kit for the selective preparation of soluble (hydrophilic) and insoluble (hydrophobic) proteins from mammalian tissues and cells, plants, yeast, bacteria, and other biological samples. The kit comes with reagents necessary for fractionation of soluble and insoluble fractions, including a strong chaotropic extraction buffer to solubilise difficult proteins.

Supplier: G-Biosciences

The Nuclear and Cytoplasmic Extraction kit is for the enrichment of cytoplasmic and nuclear fractions from cultured cells and tissues. The kit generates a cleaner separation of cytoplasmic proteins from nuclear proteins and is ideal for expression and transport studies.

Supplier: Merck Millipore (Novagen)

BugBuster® Plus Lysonase™ includes BugBuster Protein Extraction Reagent and Lysonase Bioprocessing Reagent, an optimised, ready-to-use blend of rLysozyme™ and Benzonase® Nuclease in a single kit. This powerful combination of two reagents allows maximum recovery of active soluble protein from both Gram-negative and Gram-positive bacteria. The BugBuster® and rLysozyme™ efficiently lyse the bacterial cells while the Benzonase® degrades nucleic acids to facilitate downstream processing. Requires 5 ml BugBuster and 10 µl Lysonase per 1 g cell paste. The kits provide sufficient reagents for protein extraction from 20 g or 100 g cell paste.

Supplier: Abcam

Mitochondria isolation kit for cultured cells is designed for efficient isolation of mitochondria from cultured mammalian cells. The kit provides enough reagents for up to 20 isolations and is suitable for analyzing mitochondria by Western blotting, immunocapture, or activity assays. - Cited in over 75 publications - Isolates mitochondria from cultured mammalian cells with ease - Useful in Western blotting, immunocapture, or mitochondrial activity assays - Sufficient reagents for up to 20 benchtop mitochondria isolations

Supplier: Abcam

Mitochondria isolation kit for tissue is designed for the efficient isolation of mitochondria from hard or soft tissues, providing high-quality mitochondria for downstream analysis. The kit contains enough reagents for up to 10 isolations. - Cited in over 65 publications - Isolates mitochondria from both hard and soft tissues, such as brain, liver, and heart - Suitable for western blotting, immunocapture, or activity assays - Simple Protocol – no need for ultracentrifugation, making the process more efficient and user-friendly

Supplier: Abcam

Abcam's Plant Chromatin Extraction Kit (ab156906) is a complete set of optimized buffers and reagents for isolating chromatin or DNA-protein complexes from plants in a simple and rapid format.

Supplier: Abcam

60-minute chromatin extraction kit is a complete set of optimized buffers and reagents for isolating chromatin or DNA-protein complex from mammalian cells and tissues in a simple and rapid format. - Cited in over 50 publications

Supplier: Thermo Fisher Scientific

Pierce™ TMT™ Isobaric Mass Tagging kits and reagents enable multiplex quantitation of proteins extracted from cells and tissues using tandem mass spectrometry. The Tandem Mass Tag (TMT) reagent family consists of TMTduplex and TMTsixplex sets which are specially designed to enable a rapid and cost-effective transition from method development to high-throughput protein quantitation.

Supplier: G-Biosciences

FOCUS™ Glycoprotein kit is based on lectin binding of specific glycoproteins with terminal α-D mannosyl and α-D glycosyl proteins.

Supplier: Abcam

Lipid Extraction Kit (Chloroform-Free) provides a safer, chloroform-free method for efficient lipid isolation from plasma, serum, tissue, and cells. The recovered organic phase can be dried and resuspended for downstream lipid analysis. - Cited in over 15 publications - Supports multiple sample types including - plasma, serum, tissue, suspension cells, and adherent cells. - Chloroform-free - eliminates the use of toxic chloroform in lipid extraction - Efficient lipid isolation – high-yield lipid extraction with minimal contamination - Sufficient for 50 lipid preparations (25 µL sample size)

Supplier: Enzo Life Sciences

Rapid, reliable method to isolate histone proteins in mammalian cells and tissues for histone modification applications.

Supplier: G-Biosciences

A reagent kit for detection and estimation of SDS in a sample. Mix the test sample in the extraction buffer reagents provided with the kit. If SDS is present in the sample, a blue colour is extracted that can be quantitatively measured.

Supplier: G-Biosciences

FOCUS™ PhosphoRich™ is a ready-to-use kit that enriches phosphorylated proteins and phosphopeptides from complex biological samples. The kit contains spin columns that have a phosphoprotein binding resin with a binding capacity of ~20 mg phosphorylated ovalbumin per column. The resin columns supplied with the kit can be reused, if regenerated and stored properly. FOCUS™ PhosphoRich™ is suitable for 2D gel electrophoresis and for proteomics and cell signalling studies.

Supplier: G-Biosciences

Antibody Fab Fragmentation Kits are designed for the generation and isolation of Fab fragments from IgG molecules. The kits utilise Immobilised Papain resin. Immobilised Papain offers the advantage of generating Fab and fragments without the need to remove the papain enzyme after digestion. Following papain digestion the Fab fragments are separated from undigested IgG and the Fc region with the supplied Protein A Spin Column. Protein A Resin binds the IgG and Fc molecules and the Fab are rapidly collected.

Supplier: Enzo Life Sciences

Rapid, reliable method to isolate nuclear proteins in mammalian cells and tissues.

Supplier: Abcam

5 cells or 1 mg of tissue, with optimal results from >10^6 cells or >10 mg of tissue - High yield – up to 0.4 mg of histone protein per 10^7 cells or 100 mg of tissue - Useful in western blot analysis of histone modifications and post-translational modifications - Not for ChIP; use a chromatin extraction kit for ChIP preparation

Supplier: G-Biosciences

The F(ab)₂ Fragmentation kits are designed for the generation and isolation of F(ab)₂ fragments from IgG molecules. The kit utilises Immobilised Pepsin resin. Pepsin is a proteolytic enzyme that is routinely used for the generation of F(ab)₂ fragments from immunoglobulin G (IgG). One or more of the disulfide bonds that join the heavy chains in the hinge region are preserved, so the two Fab regions of the antibody remain joined together, yielding a divalent molecule (containing two antibody binding sites), hence the designation F(ab)₂. The light chains remain intact and attached to the heavy chain, whereas the Fc fragment is digested into small peptides. The Immobilised Pepsin offers the distinct advantage of eliminating enzyme contamination of the F(ab)₂ fragments.

Supplier: Abcam

ab110171 contains sufficient reagents for up to 20 benchtop mitochondria isolations from mammalian cultured cells.

Supplier: Enzo Life Sciences

Rapid, reliable, high throughput method to isolate histone proteins in mammalian cells for histone modification applications.

Supplier: Enzo Life Sciences

Rapid, reliable method to isolate nucleic acid-free nuclear proteins in mammalian cells and tissues.

Supplier: G-Biosciences

FOCUS™ SubCell for the fractionation of mitochondrial, nuclear, and cytosolic soluble and membrane proteins.

Supplier: G-Biosciences

Lipid rafts are membrane microdomains that are enriched in caveolin, cholesterol, glycolipids, sphingolipids and glycosyl-phosphatidylinositol. Lipid rafts are also known as detergent-insoluble glycolipid-enriched complexes (GEMs) or DIGs. Many signalling proteins, including glycosylphosphatidylinositol (GPI)-anchored proteins, doubly-acylated tyrosine kinases of the Src family, and transmembrane proteins, are located in lipid rafts. Lipid raft localised proteins have been shown to be involved in intracellular membrane trafficking and signalling.

Supplier: Thermo Fisher Scientific

B-PER™ Complete Bacterial Protein Extraction Reagent is an all-in-one formulation of non ionic detergent and enzymes that enables mild extraction of proteins from both gram-negative and gram-positive bacteria without the need for mechanical disruption. B-PER Complete Reagent combines a universal nuclease and lysozyme to improve cell lysis and reduce viscosity, and may be used for soluble protein extraction and inclusion body purification from bacterial cell lysates.

Supplier: Biotium

AccuOrange™ Protein Quantitation Kit is a highly sensitive fluorescence-based assay for quantitating purified protein samples in 96-well format (excitation/emission = 480/598 nm).