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Accurately quantifying total protein concentration is a key to most experiments and is often necessary for isolation, separation, and analysis of proteins by biochemical methods. When it is necessary to determine the total protein concentration in a sample, one of the first factors to consider is the selection of a protein assay method. These choices are typically based upon the compatibility of the method with the samples to be assayed. Each method has its own advantages and disadvantages.
Pierce™ 660 nm Protein Assay Kit and Reagent
Supplier: Thermo Fisher Scientific
Pierce™ 660 nm Protein Assay is a ready to use, detergent- and reducing agent-compatible assay reagent to quickly measure (A660 nm) total protein concentration compared to a protein standard.
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Allergen protein rapid test kits
Supplier: 3M Food Safety
The 3M™ Allergen Protein Rapid Kits are intended for screening for the presence of specific proteins in clean-in-place (CIP) final rinse water, environmental swab samples, food ingredients and processed food products.
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Compat-Able™ BCA™ Protein assay kit
Supplier: Thermo Fisher Scientific
The Compat-Able™ Reagents remove salts, detergents, reducing agents and other substances from protein samples to eliminate interference with protein assays. The protocol selectively precipitates all of the protein, thereby allowing the non-protein components to be easily decanted and removed. The purified, precipitated protein is redissolved in water, making it ready for accurate determination of the protein concentration by standard protein assay methods. The procedure is accomplished in less than 10 min with just a few simple steps. The Compat-Able™ Preparation Set is available conveniently bundled with the Pierce BCA™ Protein Assay Kit.
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Pierce™ Biotin Quantitation Kit
Supplier: Thermo Fisher Scientific
Pierce™ Biotin Quantitation Kit includes aliquots of HABA reagent and biotinylated protein standard for convenient colorimetric determination of biotinylation levels in labeled antibodies and other proteins.
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Firefly luciferase glow assay kit, Pierce™
Supplier: Thermo Fisher Scientific
The Pierce™ Firefly Luciferase Glow Assay Kit provides a bright and stable bioluminescence signal (half-life greater than 2 hours) in the presence of firefly luciferase reporters for protein expression assays.
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ApoSight™ Green Caspase 3/7 substrate
Supplier: AAT BIOQUEST
ApoSight™ Green Caspase 3/7 substrate is the first fluorogenic probe for the direct fluorescence imaing of caspase activities in live cells.
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ReadiUse™ TCEP Removal Solution
Supplier: AAT BIOQUEST
TCEP is a common reducing reagent widely used to break disulfide bonds within and between proteins.
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GST protein interaction pull down kit, Pierce™
Supplier: Thermo Fisher Scientific
The Pierce™ GST Interaction Pull-Down Kit contains the necessary components to capture and purify proteins that interact with GST-tagged fusion proteins.
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Amplite® Rapid Colorimetric Biotin Quantitation Kit
Supplier: AAT BIOQUEST
Optimised to use with Nanodrop. Amplite™ colorimetric biotin quantitation kit is designed to quantify the number of biotins attached to a protein or other macromolecule with a nanodrop spectrometer.
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Protein Assay, Protn-Latex™
Supplier: G-Biosciences
A simple and reliable method for estimation of protein contamination in latex. Detects as low as 25 to 40 μg protein/gm latex (or 25 to 50 ppm). Protein solutions applied to the test strips form compact and symmetrical spots, their diameter being directly proportional to protein concentration. Protn-Latex™ latex protein extraction buffer extracts protein from the latex sample, which is then applied to the test strips and rapidly developed. By measuring the Ø of protein spots with the Protn-Latex™ card, the range of protein concentration in the sample can be determined.
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Non-interfering Protein Assay™ kit, Calbiochem®
Supplier: Merck Millipore (Calbiochem)
Non-Interfering Protein Assay™ Kit is an easy-to-use protein assay that overcomes interference of agents found in protein solutions including detergents, chelating agents, reducing agents, amines, sugars, urea, and others. The Universal Protein Precipitating Agent (UPPA™ Reagent) is used to precipitate and immobilize the protein in the tube while the interfering reagents are removed.
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Quantitative colorimetric peptide assay, Pierce™
Supplier: Thermo Fisher Scientific
Thermo Scientific™ Pierce™ Quantitative Colorimetric Peptide Assay is an easy-to-use colorimetric microplate assay designed specifically to improve the sensitivity and reproducibility of quantitation of peptide mixtures.
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Streptavidin poly-HRP conjugate, Pierce™
Supplier: Thermo Fisher Scientific
Pierce™ Streptavidin Poly-HRP conjugate is biotin-binding protein conjugated with polymers of horseradish peroxidase, enabling signal amplification and detection of biotinylated antibodies for IHC and other methods.
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Glutathione Fluorometric Assay Kit
Supplier: Abcam
Simple assay to detect total glutathione.
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Biotin labelling kit
Supplier: Abnova
Biotin Labelling Kit is used for simple and rapid preparation of biotin-labeled antibodies and proteins.
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2-D Quant kit
Supplier: Cytiva
2-D Quant Kit is designed for the accurate determination of protein concentration in samples prepared for electrophoresis techniques such as 2-D electrophoresis, SDS-PAGE, or IEF.
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Histone, from calf thymus, MP Biomedicals
Supplier: MP Biomedicals
Histones are soluble in water (10 mg/ml) or 0,5 N HCl (10 mg/ml), yielding a clear to hazy solution depending on the fraction. They are soluble in 6 M urea (4 to 10 mg/ml), but this will denature the histones.