Either measuring substrate consumption or overall production, the enzyme assays are used to monitor enzymatic activity. Provided with the required supplies and reagents for any preferred method, enzyme kinetics and inhibition studies can be successfully completed. While continuous assay sampling methods give activity readings throughout development, discontinuous techniques calculate after the reaction has been stopped. The standardized enzyme assays will provide accurate identification and measurement of the protein molecules present in samples.
Amplite® Rapid Fluorimetric Glutathione GSH/GSSG Ratio Assay Kit *Green Fluorescence*
Supplier: AAT BIOQUEST
When cells are exposed to increased levels of oxidative stress, GSSG will accumulate and the ratio of GSH to GSSG will decrease.
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Amplite® Colorimetric Alkaline Phosphatase Assay Kit *Yellow Color*
Supplier: AAT BIOQUEST
Alkaline phosphatase is a highly sensitive enzyme for ELISA, immuno-histochemical, Northern, Southern and Western blot applications.
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Pierce™ Alkaline Phosphatase, Biotinylated
Supplier: Thermo Fisher Scientific
Biotinylated alkaline phosphatase is most commonly used in immunohistochemistry (IHC) to amplify the signal of biotinylated primary antibodies using the ABC staining method.
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Amplite® Fluorimetric cADP-Ribose Assay Kit
Supplier: AAT BIOQUEST
This cADP-ribose assay can be performed in a convenient 96-well or 384-well microtiter plate.
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Amplite® Fluorimetric Endotoxin Detection Kit
Supplier: AAT BIOQUEST
Lipopolysaccharide (LPS), also known as endotoxin, is the major component of the outer membrane of Gram-negative bacteria.
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CPRG assay kit
Supplier: OZ BIOSCIENCES
CPRG assay kit is designed to quantify low expression level β-Galactosidase.
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Amplite® Fluorimetric Lead (II) Ion Quantitation Kit
Supplier: AAT BIOQUEST
Lead is a highly poisonous metal to human beings, especially young children.
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Pierce™ Firefly Luc One-Step Glow Assay Kit
Supplier: Thermo Fisher Scientific
The Thermo Scientific Pierce Firefly One-Step Luc Glow Assay Kit measures firefly luciferase activity in mammalian cell culture using a single reagent-addition step, making it ideal for high-throughput screening (HTS) applications.
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Amplite® Fluorimetric DPP4 Activity Assay Kit
Supplier: AAT BIOQUEST
Designed for consistent and dependable results. The Amplite® fluorimetric DPP4 activity assay kit provides a simple, quick, and direct protocol for measuring DPP4 Activity.
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Amplite® Fluorimetric DPP4 Inhibitor Screening Kit
Supplier: AAT BIOQUEST
Designed for consistent and dependable results. The Amplite® Fluorimetric DPP4 Inhibitor Screening Kit provides an efficient and direct method for evaluating potential DPP4 inhibitors.
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Gaussia luciferase glow assay kit, Pierce™
Supplier: Thermo Fisher Scientific
The Thermo Scientific Pierce Gaussia Luciferase Glow Assay kit provides an extremely bright and stable bioluminescence signal (half-life greater than one hour), especially in the presence of Gaussia-Dura luciferase reporter.
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Pierce™ Cypridina Luciferase Glow Assay Kit
Supplier: Thermo Fisher Scientific
The Thermo Scientific Pierce Cypridina Luciferase Glow Assay Kit provides an extremely bright and stable bioluminescence signal (half-life greater than 1 hour) in the presence of Cypridina luciferase reporters.
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Amplite® Colorimetric L-Aspartate (Aspartic Acid) Assay Kit
Supplier: AAT BIOQUEST
Aspartate (or Aspartic acid) is a negatively charged, polar amino acid.
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Amplite® Colorimetric Gama-Glutamyltransferase (GGT) Activity Assay Kit
Supplier: AAT BIOQUEST
Designed for consistent and dependable results. The Amplite® Colorimetric Gamma-Glutamyltransferase (GGT) Activity Assay Kit provides an efficient and straightforward method for measuring GGT activity across various sample types.
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Amplite® Fluorimetric HDAC Activity Assay Kit *Green Fluorescence*
Supplier: AAT BIOQUEST
Histone deacetylases (HDAC) are a class of enzymes that remove acetyl groups from a ε-N-acetyl lysine amino acid on a histone.
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Pierce™ Gaussia Luciferase Flash Assay Kit
Supplier: Thermo Fisher Scientific
The Thermo Scientific Pierce Gaussia Luciferase Flash Assay Kit provides researchers with the most sensitive assay for transcriptional activity of regulatory elements in mammalian cell culture media and whole cell lysate.
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ONPG assay kit
Supplier: OZ BIOSCIENCES
ONPG assay kit is ideal to quantify high expression level of beta-Gal.
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Serine hydrolase probes, ActivX™ FP
Supplier: Thermo Fisher Scientific
The ActivX® FP serine hydrolase probes enable selective labeling and enrichment of active serine hydrolases. Fluorophosphonate (FP) probes specifically label serines of enzymatically active serine hydrolases. FP probes are also effective for screening small molecule inhibitors against enzymes derived from cell lysates, subcellular fractions, tissues and recombinant proteins. Depending on the active-site probe tag group and the enzyme class to be profiled, active site-labeled enzymes are detected and quantified by Western blot, fluorescent gel imaging or mass spectrometry.
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SAM Methyltransferase assay, SAMfluoro™
Supplier: G-Biosciences
The SAMfluoro™ assay is a fluorescent, continuous enzyme coupled assay that can continuously monitor SAM-dependent methyltransferases without the use of radioactive labels or endpoint measurements.
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Peroxide assay kit, PEROXsay™
Supplier: G-Biosciences
PEROXsay™ is a colorimetric quantitative peroxide assay that measures the oxidation of ferrous (Fe²⁺+) ions to ferric (Fe³⁺) ions. Peroxides react with a sugar alcohol converting it to a peroxyl radical that subsequently starts the oxidation of ferrous ions to ferric ions. The acidic pH of the assay allows the ferric (Fe³⁺) ion to complex with the colorimetric reagent, resulting in a change in absorbance that is proportional to the peroxide concentration.
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Amplite® Colorimetric L-Lactate Dehydrogenase (LDH) Assay Kit
Supplier: AAT BIOQUEST
Lactate dehydrogenase (LDH) is an oxidoreductase enzyme that catalyses the interconversion of pyruvate and lactate.
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Amplite® Fluorimetric Pyruvate Assay Kit
Supplier: AAT BIOQUEST
Pyruvate is an important chemical compound in intracellular metabolic pathways.
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Amplite® Fluorimetric Glycerol 3-Phosphate (G3P) Assay Kit
Supplier: AAT BIOQUEST
Glycerol 3-Phosphate (G3P) is an important intermediate in the glycolysis metabolic pathway.
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Amplite® Fluorimetric NADH Assay Kit *Red Fluorescence*
Supplier: AAT BIOQUEST
Nicotinamide adenine dinucleotide (NAD+) and nicotinamide adenine dinucleotide phosphate (NADP+) are two important cofactors found in cells.
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MMP-3 Green™ Substrate
Supplier: AAT BIOQUEST
The matrix metalloproteinases (MMPs) constitute a family of zinc-dependent endopeptidases that function within the extracellular matrix.
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Amplite® Fluorimetric Protein Quantitation Kit *Orange Fluorescence*
Supplier: AAT BIOQUEST
Protein quantification is an essential task in protein purification, electrophoresis, cell biology, molecular biology and other research applications.
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Amplite® Red Peroxidase Substrate
Supplier: AAT BIOQUEST
Amplite® Red is a sensitive fluorogenic peroxidase substrate that generates a highly red fluorescent product that has maximum absorption of 571 nm and maximum emission of 585 nm.
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Amplite® Fluorimetric Aldehyde Quantitation Kit
Supplier: AAT BIOQUEST
The formation, reactivity and toxicity of aldehydes originating from the peroxidation of lipids of cellular membranes have received great attention in recent years.
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Amplite® Fluorimetric Sphingomyelinase Assay Kit *Red Fluorescence*
Supplier: AAT BIOQUEST
Five types of sphingomyelinase (SMase) have been identified based on their cation dependence and pH optima of action.
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Amplite® Fluorimetric Glucose-6-Phosphate Dehydrogenase (G6PD) Assay Kit
Supplier: AAT BIOQUEST
Glucose-6-phosphate dehydrogenase (G6PD) catalyses the conversion of glucose-6-phosphate to 6-phosphoglucono-δ-lactone, the first and rate-limiting step in the pentose phosphate pathway.