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272 Ergebnisse für Enzymtests

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Either measuring substrate consumption or overall production, the enzyme assays are used to monitor enzymatic activity. Provided with the required supplies and reagents for any preferred method, enzyme kinetics and inhibition studies can be successfully completed. While continuous assay sampling methods give activity readings throughout development, discontinuous techniques calculate after the reaction has been stopped. The standardized enzyme assays will provide accurate identification and measurement of the protein molecules present in samples.

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Amplite® Colorimetric D-Lactate Dehydrogenase (LDH) Assay Kit

Amplite® Colorimetric D-Lactate Dehydrogenase (LDH) Assay Kit

Supplier: AAT BIOQUEST

Lactate dehydrogenase (LDH) is an oxidoreductase enzyme that catalyses the interconversion of pyruvate and lactate.

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Amplite® Fluorimetric Glucose Oxidase Assay Kit *Red Fluorescence*

Amplite® Fluorimetric Glucose Oxidase Assay Kit *Red Fluorescence*

Supplier: AAT BIOQUEST

The glucose oxidase is a dimeric protein that catalyses the oxidation of beta-D-glucose into hydrogen peroxide and D-glucono-1,5-lactone, which is hydrolyzed to gluconic acid.

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Amplite® Colorimetric Antioxidant Activity Assay Kit

Amplite® Colorimetric Antioxidant Activity Assay Kit

Supplier: AAT BIOQUEST

Reactive oxygen species (ROS) are essential in cell signaling and maintaining physiological cell metabolism.

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Amplite® Colorimetric Caspase 3/7 Assay Kit *Yellow Color*

Amplite® Colorimetric Caspase 3/7 Assay Kit *Yellow Color*

Supplier: AAT BIOQUEST

Caspases play important roles in apoptosis and cell signaling.

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Amplite® Fluorimetric Glucose Quantitation Kit

Amplite® Fluorimetric Glucose Quantitation Kit

Supplier: AAT BIOQUEST

Glucose, a monosaccharide, is the most important carbohydrate in biology.

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Amplite® Universal Fluorimetric Kinase Assay Kit *Red Fluorescence*

Amplite® Universal Fluorimetric Kinase Assay Kit *Red Fluorescence*

Supplier: AAT BIOQUEST

The Amplite® Universal Kinase Assay Kit is based on the monitoring of ADP formation, which is directly proportional to enzyme phoshphotransferase activity and is measured fluorimetricaly. This kit provides a fast, simple, and homogeneous assay for measure kinases activities. The characteristics of its high sensitivity (<0.2 uM ADP), broad ATP tolerance (1-300 uM), non-antibody based, non-radioactive and no-wash method to detect the amount of ADP produced as a result of enzyme activity make it an ideal kit for determining kinase Michaelis-Menten kinetics and for screening and identifying kinase inhibitors. The assay can be performed in a convenient 96-well or 384-well microtiter-plate format and easily adapted to automation with no separation steps required.

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Amplite® Colorimetric Beta-Galactosidase Assay Kit

Amplite® Colorimetric Beta-Galactosidase Assay Kit

Supplier: AAT BIOQUEST

E. coli beta-galactosidase is a 464 kD tetramer.

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Amplite® IR

Amplite® IR

Supplier: AAT BIOQUEST

Our Amplite® IR is a fluorogenic peroxidase substrate that generates near infrared fluorescence upon reaction with peroxidase and H2O2.

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Amplite® Fluorimetric Glutamic Acid Assay Kit *Red Fluorescence*

Amplite® Fluorimetric Glutamic Acid Assay Kit *Red Fluorescence*

Supplier: AAT BIOQUEST

Glutamic acid is one of the 20 proteinogenic amino acids.

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Amplite® Fluorimetric Glutathione Assay Kit *Green Fluorescence*

Amplite® Fluorimetric Glutathione Assay Kit *Green Fluorescence*

Supplier: AAT BIOQUEST

The monitoring of reduced and oxidised glutathione (GSH) in biological samples is essential for evaluating the redox and detoxification status of cells and tissues in relation to the protective role of glutathione against oxidative and free-radical-mediated cell injury.

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Amplite® Fluorimetric NADP/NADPH Ratio Assay Kit *Red Fluorescence*

Amplite® Fluorimetric NADP/NADPH Ratio Assay Kit *Red Fluorescence*

Supplier: AAT BIOQUEST

Nicotinamide adenine dinucleotide (NAD⁺) and nicotinamide adenine dinucleotide phosphate (NADP⁺) are two important cofactors found in cells.

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ReadiUse™ NADPH Regenerating Kit

ReadiUse™ NADPH Regenerating Kit

Supplier: AAT BIOQUEST

NADPH provides the reducing equivalents for biosynthetic reactions and for oxidation-reduction involved in protection against the toxicity of ROS (reactive oxygen species).

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Amplite® Fluorimetric NADP Assay Kit *Blue Fluorescence*

Amplite® Fluorimetric NADP Assay Kit *Blue Fluorescence*

Supplier: AAT BIOQUEST

Nicotinamide adenine dinucleotide (NAD+) and nicotinamide adenine dinucleotide phosphate (NADP+) are two important cofactors for many enzyme reactions found in living cells.

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Amplite® Fluorimetric Peroxidase (HRP) Assay Kit *Red Fluorescence*

Amplite® Fluorimetric Peroxidase (HRP) Assay Kit *Red Fluorescence*

Supplier: AAT BIOQUEST

Peroxidase is a small molecule (MW ~40 KD) that can usually be conjugated to an antibody in a 4:1 ratio.

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Amplite® Luminometric Peroxidase (HRP) Assay Kit

Amplite® Luminometric Peroxidase (HRP) Assay Kit

Supplier: AAT BIOQUEST

Peroxidase is a small molecule (MW ~40 KD) that can usually be conjugated to an antibody in a 4:1 ratio.

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Amplite® Colorimetric L-Lactate Assay Kit

Amplite® Colorimetric L-Lactate Assay Kit

Supplier: AAT BIOQUEST

Lactic acid is chiral and has two optical isomers: L-lactic acid and D-lactic acid.

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Amplite® Colorimetric Calcium Quantitation Kit  *Blue Color*

Amplite® Colorimetric Calcium Quantitation Kit *Blue Color*

Supplier: AAT BIOQUEST

Calcium is essential for all living organisms, particularly in cell physiology, where movement of the calcium ion Ca²⁺ into and out of the cytoplasm functions as a signal for many cellular processes.

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CPRG assay kit

CPRG assay kit

Supplier: OZ BIOSCIENCES

CPRG assay kit is designed to quantify low expression level β-Galactosidase.

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Gaussia luciferase glow assay kit, Pierce™

Gaussia luciferase glow assay kit, Pierce™

Supplier: Thermo Fisher Scientific

The Thermo Scientific Pierce Gaussia Luciferase Glow Assay kit provides an extremely bright and stable bioluminescence signal (half-life greater than one hour), especially in the presence of Gaussia-Dura luciferase reporter.

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Pierce™ Cypridina Luciferase Glow Assay Kit

Pierce™ Cypridina Luciferase Glow Assay Kit

Supplier: Thermo Fisher Scientific

The Thermo Scientific Pierce Cypridina Luciferase Glow Assay Kit provides an extremely bright and stable bioluminescence signal (half-life greater than 1 hour) in the presence of Cypridina luciferase reporters.

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Amplite® Luciferase Reporter Gene Assay Kit *Bright Glow*

Amplite® Luciferase Reporter Gene Assay Kit *Bright Glow*

Supplier: AAT BIOQUEST

Common reporter genes include beta-galactosidase, beta-glucuronidase and luciferase.

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Amplite® Fluorimetric Lead (II) Ion Quantitation Kit

Amplite® Fluorimetric Lead (II) Ion Quantitation Kit

Supplier: AAT BIOQUEST

Lead is a highly poisonous metal to human beings, especially young children.

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Amplite® Colorimetric Xanthine Oxidase Assay Kit

Amplite® Colorimetric Xanthine Oxidase Assay Kit

Supplier: AAT BIOQUEST

Xanthine oxidase (XO)is an enzyme that catalyzes the oxidation of hypoxanthine to xanthine and can further catalyse the oxidation of xanthine to uric acid.

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Amplite® Human Serum Albumin (HSA) Site I Binding Assay Kit

Amplite® Human Serum Albumin (HSA) Site I Binding Assay Kit

Supplier: AAT BIOQUEST

Human serum albumin (HSA) is one of the most important carriers for acidic drugs in human plasma and has been shown to bind a large number of different compounds in a reversible manner.

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Amplite® Fluorimetric D-Lactate Assay Kit

Amplite® Fluorimetric D-Lactate Assay Kit

Supplier: AAT BIOQUEST

Lactic acid is chiral and has two optical isomers: L-lactic acid and D-lactic acid.

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Amplite® Fluorimetric L-Aspartate (Aspartic Acid) Assay Kit

Amplite® Fluorimetric L-Aspartate (Aspartic Acid) Assay Kit

Supplier: AAT BIOQUEST

Aspartate (or Aspartic acid) is a negatively charged, polar amino acid.

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Amplite® Fluorimetric Beta-Hydroxybutyrate (Ketone Body) Assay Kit

Amplite® Fluorimetric Beta-Hydroxybutyrate (Ketone Body) Assay Kit

Supplier: AAT BIOQUEST

Ketone bodies are produced by the liver and used peripherally as an energy source when blood glucose levels drop.

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Amplite® Colorimetric  Glycerol 3-Phosphate (G3P) Assay Kit

Amplite® Colorimetric Glycerol 3-Phosphate (G3P) Assay Kit

Supplier: AAT BIOQUEST

Glycerol 3-Phosphate (G3P) is an important intermediate in glycolysis metabolic pathway.

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Amplite® Colorimetric Xanthine Assay Kit

Amplite® Colorimetric Xanthine Assay Kit

Supplier: AAT BIOQUEST

Xanthine is a purine base found in most human body tissues and fluids.

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Amplite® Fluorimetric Xanthine Assay Kit

Amplite® Fluorimetric Xanthine Assay Kit

Supplier: AAT BIOQUEST

Xanthine is a purine base found in most human body tissues and fluids.

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