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Zelltests

Zelltests

Zelluläre Assays waren schon immer ein leistungsstarkes Werkzeug im Forschungslabor. Sie sind äußerst vielseitig und können so konzipiert werden, dass sie nahezu jede zelluläre oder biochemische Funktion messen. Die empfindliche Detektion fluoreszierender Sonden unterstützt die Bewertung von Zellsignalisierung und Zelltodwegen, was für die schnelle Quantifizierung der Zellviabilität nützlich ist. Zelluläre Assays können verwendet werden, um die Auswirkungen verschiedener Behandlungen auf die Zellviabilität zu messen und toxische Effekte zu bewerten, die über eine Vielzahl von Mechanismen vermittelt werden.

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Firefly and Renilla Luciferase single tube Assay

Firefly and Renilla Luciferase single tube Assay

Supplier: Biotium

This kit is designed to measure Firefly and Renilla luciferase activities in transfected cell reporter assays. Firefly luciferase is widely used as a reporter for studying gene regulation and function, and for pharmaceutical screening. It is a very sensitive genetic reporter due to the absence of endogenous luciferase activity in mammalian cells or tissues. Renilla luciferase has been used as a reporter gene for studying gene regulation and function in vitro and in vivo. It commonly is used in multiplex transcriptional reporter assays or as a normalising transfection control for firefly luciferase assays.

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Alamar blue cell viability assay

Supplier: G-Biosciences

AlamarBlue Cell Viability Assay reagent quantitatively measures the proliferation of mammalian cell lines, bacteria and fungi. The dye incorporates an oxidation-reduction (REDOX) indicator that both fluoresces and change colour in response to the chemical reduction of growth medium due to cell growth.

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Yeast viability staining kits

Yeast viability staining kits

Supplier: Biotium

The Yeast Viability Staining Kit is a fluorescent assay to determine yeast viability. Each kit contains two fluorescent components: CF® Dye-labeled Concanavalin A (Con A) and Live-or-Dye™ viability dye. Biotium offers 3 different color combinations to choose from.

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Amyloid-Plaque-Färbereagenz, mit EtBr-Gegenfärbung, Amylo-Glo® RTD™

Amyloid-Plaque-Färbereagenz, mit EtBr-Gegenfärbung, Amylo-Glo® RTD™

Supplier: Biosensis

Amylo-Glo RTD ist ein Reagenz zur Färbung von Amyloid-Plaques in Gewebeschnitten.

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Annexin V-FITC Apoptose-Detektions-Kit II, Sigma-Aldrich®

Supplier: Merck Millipore (Oncogene)

Das Calbiochem® Annexin V-FITC Apoptose-Detektions-Kit II ist ein nicht-isotopisches System, das zur Detektion von Phosphatidylserin auf der äußeren Schicht der Zellmembran apoptotischer Zellen mittels Durchflusszytometrie oder Fluoreszenzmikroskopie verwendet werden kann.

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BCA protein assay kit

Supplier: Thermo Fisher Scientific

BCA Protein Assay Kit can be used to measure very dilute protein concentrations in very small sample volumes. The kit accurately measures protein concentrations from 0,5 to 30 μg/ml in tube assays and 1 to 20 μg/ml in 96- or 384-well plate assays. It is used for studying protein:protein interactions, measuring column fractions after affinity chromatography, estimating percent recovery of membrane proteins from cell extracts and high-throughput screening of fusion proteins.

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Live-or-Dye NucFix™ red staining kit

Supplier: Biotium

Live-or-Dye NucFix™ Red is a red fluorescent dye to covalently label dead cells, allowing cells with permeable plasma membranes to be detected by flow cytometry or fluorescence microscopy.

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Yeast vitality staining kit

Yeast vitality staining kit

Supplier: Biotium

A fluorescent vitality dye for yeast, identical in structure to the dye FUN® 1. Living yeast cells show red fluorescent vacuolar tubules.

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Nrf2 transcription factor assay kit

Nrf2 transcription factor assay kit

Supplier: Cayman Chemical

Nrf2 Transcription Factor Assay kit is a 96-well assay for measurement of Nrf2.

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Red/green (FUN®1), ViaVac™

Red/green (FUN®1), ViaVac™

Supplier: Biotium

A fluorescent vitality dye for yeast, identical in structure to the dye FUN® 1. Living yeast cells show red fluorescent vacuolar tubules.

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CellBrite™ Fix membrane stains, Biotium

CellBrite™ Fix membrane stains, Biotium

Supplier: Biotium

CellBrite™ Fix Farbstoffe sind fluorogene Membran-Farbstoffe, die die Plasmamembran in lebenden Zellen kovalent färben. Sie sind einzigartig unter den Membran-Farbstoffen, indem sie sowohl der Fixierung als auch der Permeabilisierung durch Reinigungsmittel standhalten können.

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Firefly luciferase assay kit

Supplier: Biotium

Firefly luciferase is widely used as a reporter for studying gene regulation and function, and for pharmaceutical screening. It is a very sensitive genetic reporter due to the absence of endogenous luciferase activity in mammalian cells or tissues.

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Live-or-Dye™ Yeast fixable live/dead staining kit

Live-or-Dye™ Yeast fixable live/dead staining kit

Supplier: Biotium

The Yeast Live-or-Dye™ Fixable Live/Dead Staining Kit is a fixable, fluorescent assay to determine yeast viability. The kit contains two fluorescent components, Thiazole Orange and Live-or-Dye™ 568/583.

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Cell proliferation assay kit, WST-1

Cell proliferation assay kit, WST-1

Supplier: Abnova

WST-1 cell proliferation assay kit provides an easy to use tool for studying induction and inhibition of cell proliferation in any in vitro model.

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Antioxidant assay kit

Antioxidant assay kit

Supplier: Abnova

Antioxidant Assay Kit is a quantitative colorimetric determination of total antioxidant capacity of cellular samples.

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ATP assay kit

ATP assay kit

Supplier: Abnova

ATP Assay Kit is for the rapid bioluminescent determination of ATP in biological samples.

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