Protein linking and labelling systems provide a convenient means for attaching fluorescent labels or biotins to antibodies and larger proteins. These kits are ideal for use with multiple applications, including flow cytometry, fluorescent microscopy, immunohistochemistry, primary detection, ELISAs, immunocytochemistry, and FISH. These systems are available with a variety of dye colours, biotin, fluorescent agents, and all the components needed to perform multiple conjugations and purifications. Protein labelling follows a simple protocol of reaction, separation, and then use.
ReadiLink™ Rapid trFluor™ Eu Antibody Labeling Kit "Microscale Optimised for Labeling 50 µg Antibody Per Reaction" (2 Labelings)
Supplier: AAT Bioquest
Many biological compounds present in cells, serum or other biological fluids are naturally fluorescent, and thus the use of conventional, prompt fluorophores leads to serious limitations in assay sensitivity due to the high background caused by the autofluorescence of the biological molecules to be assayed.
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ReadiLink™ Rapid mFluor™ Violet 450 Antibody Labelling Kit 'Microscale Optimised for Labelling 50 µg Antibody Per Reaction' (2 Labellings)
Supplier: AAT Bioquest
AAT Bioquest's mFluor™ dyes are developed for flow cytometry-focused applications.
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ReadiLink™ Rapid mFluor™ Violet 420 Antibody Labeling Kit "Microscale Optimized for Labeling 50 µg Antibody Per Reaction" (2 Labelings)
Supplier: AAT Bioquest
AAT Bioquest's mFluor™ dyes are developed for flow cytometry-focused applications.
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ReadiLink™ Rapid mFluor™ Violet 540 Antibody Labeling Kit "Microscale Optimized for Labeling 50 µg Antibody Per Reaction" (2 Labelings)
Supplier: AAT Bioquest
AAT Bioquest's mFluor™ dyes are developed for flow cytometry-focused applications.
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ReadiLink™ DIG (Digoxigenin) Nick Translation dsDNA-Markierungskit
Supplier: AAT Bioquest
Es ist mit einer Vielzahl von Probenmaterialien kompatibel, einschließlich bakterieller künstlicher Chromosomen-DNA (BAC), menschlicher genomischer DNA, gereinigten PCR-Produkten sowie superspiralisierter und linearisierter Plasmid-DNA.
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ReadiLink™ Biotin Nick Translation dsDNA-Markierungskit
Supplier: AAT Bioquest
Es ist mit einer Vielzahl von Probenmaterialien kompatibel, einschließlich bakterieller künstlicher Chromosomen-DNA (BAC), menschlicher genomischer DNA, gereinigten PCR-Produkten sowie superspiralisierter und linearisierter Plasmid-DNA.
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ReadiLink™ iFluor® 555 FISH Fluorescence Imaging Kit
Supplier: AAT Bioquest
The kit provides Taq DNA polymerase enzyme which incorporates iFluor® 555-dUTPs in the target DNA through Polymerase Chain Reaction (PCR).
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ReadiLink™ Rapid Cy3 Antibody Labelling Kit 'Microscale Optimised for Labelling 50 µg Antibody Per Reaction' (2 Labellings)
Supplier: AAT Bioquest
Cy3 is one of the most popular fluorescent labelling dyes for preparing orange-red fluorescent bioconjugates.
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ReadiLink™ xtra Rapid Cy5 Antibody Labelling Kit *BSA-Compatible* (2 Labellings)
Supplier: AAT Bioquest
ReadiLink™ xtra rapid antibody labelling kits require essentially only 2 simple mixing steps without a column purification needed.
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Buccutite™ Rapid PE-Cy7 Tandem Antibody Labelling Kit
Supplier: AAT Bioquest
Production scale optimised for labelling 1 mg antibody per reaction. Buccutite™ Rapid PE-Cy7 tandem antibody labelling kits, designed for large-scale production, provide a streamlined approach for labelling antibodies with PE, APC, PerCP and iFluor® tandem dyes.
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Buccutite™ Rapid PE Antibody Labelling Kit
Supplier: AAT Bioquest
Production scale optimised for labelling 1 mg antibody per reaction. Buccutite™ Rapid PE antibody labelling kits, designed for large-scale production, offer a convenient and efficient method to label antibodies with PE, APC and iFluor® tandem dyes.
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HIS Lite™ Cy3 Tris NTA-Ni Complex
Supplier: AAT Bioquest
Cy3-Tris NTA compound is used as a sensitive fluorescent probe for detecting polyhistidine-labelled proteins in cells, solution and solid surfaces.
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iFluor® 750-Wheat Germ Agglutinin (WGA) Conjugate
Supplier: AAT Bioquest
iFluor® 750 labelled WGA exhibits a distinct far-red fluorescence characteristic, emitting maximally at 779 nm.
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Helixyte™ iFluor® 647 Nucleic Acid Labelling Dye *Optimised for Labelling 2×100 μg DNA/RNA*
Supplier: AAT Bioquest
Helixyte™ iFluor® 647 Nucleic Acid labelling Dye is a key member of our enabling Helixyte™ nucleic acid labelling and conjugation technology.
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Sulfhydryl reactive biotin reagents, HOOK™
Supplier: G-Biosciences
HOOK™-Biotin-BM, 50 mg
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Pierce™ ABC Staining Kits
Supplier: Thermo Fisher Scientific
ABC Staining Kits include reagents for the avidin-biotin complex (ABC) technique, a highly sensitive method for immunohistochemical detection with biotinylated secondary antibodies.
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Controlled Protein-Protein Crosslinking Kit, Pierce™
Supplier: Thermo Fisher Scientific
The Pierce™ Controlled Protein-Protein Crosslinking Kit contains essential crosslinkers, modification reagents and buffers for conjugating antibodies, enzymes and other purified proteins needed as probes.
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Protein labelling reagent, iodoTMTzero™
Supplier: Thermo Fisher Scientific
The iodoTMTzero™ label reagent can be used as an isotopic 'light' duplex tag for MS-level protein quantitation.
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Immobilised reductant column
Supplier: Thermo Fisher Scientific
Pierce™ Immobilised reductant column contains beaded resin to which an thiol-based reducing agent has been immobilised to enable solid-phase reduction of peptide and protein disulphide bonds.
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MS-kompatibles magnetisches IP-Kit, Streptavidin, Pierce™
Supplier: Thermo Fisher Scientific
Das Thermo Scientific™ Pierce™ MS-kompatible magnetische IP-Kit (Streptavidin) bietet massenspektrometriefreundliche Reagenzien und ein optimiertes Protokoll für eine hocheffektive und effiziente Immunpräzipitation und Co-Immunpräzipitation von Zielantigenen vor der LC-MS-Analyse.
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Helixyte™ iFluor® 488 Nucleic Acid Labelling Dye *Optimised for Labelling 2×100 µg DNA/RNA*
Supplier: AAT Bioquest
Helixyte™ iFluor® 488 Nucleic acid labelling dye is a key member of our enabling Helixyte™ nucleic acid labelling and conjugation technology.
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Helixyte™ iFluor® 750 Nucleic Acid Labelling Dye *Optimised for Labelling 2×100 µg DNA/RNA*
Supplier: AAT Bioquest
Helixyte™ iFluor® 750 Nucleic acid labelling dye is a key member of our enabling Helixyte™ nucleic acid labelling and conjugation technology.
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Pierce™, EZ-Link™ Sulfhydryl Reactive Biotinylation Reagents
Supplier: Thermo Fisher Scientific
Sulfhydryl groups are the second most common target for modification in biological studies. They are found as exposed cysteines in protein and peptides. Sulfhydryl group biotinylation provides an advantage in some applications; for example, targeting a sulfhydryl group can help preserve the biological activity of an enzyme when amines are found at the active site.
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PEGylated BM (bismaleimido) Crosslinkers, Homobifunctional, Pierce™
Supplier: Thermo Fisher Scientific
Cross linkers are used for the identification of near-neighbour protein relationships and ligand-receptor interactions. Homobifunctional, amine-reactive NHS esters or imidates, and heterobifunctional, amine-reactive, photoactivatable phenyl azides are the most commonly used cross linkers for these applications.
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PSA™ iFluor® 555 Imaging Kit with Goat Anti-Mouse IgG
Supplier: AAT Bioquest
iFluor® 555 PSA kit is a much superior replacement for Alexa Fluor 555 tyramide-based kit or other spectrally similar fluorescent tyramide or TSA kits.
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PSA™ iFluor® 647 Imaging Kit with Goat Anti-Mouse IgG
Supplier: AAT Bioquest
iFluor® 647 PSA kit is a much superior replacement for Alexa Fluor 647 tyramide-based kit or other spectrally similar fluorescent tyramide or TSA kits.
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Buccutite™ Peroxidase (HRP) Antibody Conjugation Kits (2 Labelings)
Supplier: AAT Bioquest
Protein-protein conjugations are commonly performed with a bifunctional linker (such as the commonly used SMCC), having different reactivity on each end for linking two different proteins.
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Buccutite™ Rapid PE-Texas Red Tandem Antibody Labelling Kits (2 Labellings)
Supplier: AAT Bioquest
PE-Texas Red is a popular colour used in flow cytometry.
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Buccutite™ Rapid Crosslinked APC Antibody Labelling Kit
Supplier: AAT Bioquest
Production scale optimised for labelling 1 mg antibody per reaction. Buccutite™ Rapid crosslinked APC antibody labelling kits, designed for large-scale production, offer a convenient and efficient method to label antibodies with APC, PE and iFluor® tandem dyes.
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iFluor® 790-Wheat Germ Agglutinin (WGA) Conjugate
Supplier: AAT Bioquest
iFluor® 790 labelled WGA conjugate exhibits a distinct near-infrared fluorescence characteristic, emitting maximally at 812 nm.
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