Protein linking and labelling systems provide a convenient means for attaching fluorescent labels or biotins to antibodies and larger proteins. These kits are ideal for use with multiple applications, including flow cytometry, fluorescent microscopy, immunohistochemistry, primary detection, ELISAs, immunocytochemistry, and FISH. These systems are available with a variety of dye colours, biotin, fluorescent agents, and all the components needed to perform multiple conjugations and purifications. Protein labelling follows a simple protocol of reaction, separation, and then use.

Mono-Reactive NHS Ester dye packs, Amersham CyDye™

Supplier: Cytiva

Mono-reactive dye pack contains derivatised CyDye™ that carries only one reactive group on each dye molecule for targeted and specific labelling of amine groups, for example on antibodies, peptides or oligonucleotides.

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Amersham CyDye DIGE fluors

Supplier: Cytiva

CyDye™ DIGE fluors are available as minimal and saturation labeling dyes. The minimal dyes are intended for general 2-D application use where sufficient amounts of sample are available. The saturation dyes from the Scarce Sample Labeling Kit are designed to be used for applications where only small amounts of sample are available, for example in Laser Capture Microdissection.

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Monofunctional maleimide fluors, CyDye™

Supplier: Cytiva

Cy3 and Cy5 monofunctional maleimides are particularly suitable for the selective labelling of molecules containing free sulfhydryl groups, such as cysteine residues in proteins and peptides and oligonucleotides.

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Labeling dye, Atto 488, Sigma-Aldrich®

Supplier: Merck

Atto 488 is a labeling dye with high molecular absorption (90000) and quantum yield (0,80) as well as sufficient stokes shift between excitation and emission maximum. It is optimised for excitation with an argon laser, and is characterised by high photostability.

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DNA labeling reagent, Atto 647 NHS Ester

Supplier: Merck

Atto 647 NHS ester is a reagent for the covalent labeling of molecules with Atto 647 flourescent dye.

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Atto 647N-biotin, BioReagent

Supplier: Merck

Designed for high sensitivity applications, including single molecule detection. These labels have rigid structures that do not show any cis-trans-isomerisation and display exceptional intensity with minimal spectral shift on conjugation.

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Peroxidase-Substrate, KPL TrueBlue™

Supplier: SERACARE LIFE SCIENCES

KPL TrueBlue™ Peroxidase-Substrat ist ein hochsensitives chromagenes Substrat für die Visualisierung von mit Meerrettichperoxidase markierten Reporter-Reagenzien.