Protein- Expression

Supplier: TriLink BioTechnologies

Dieses mRNA-Konstrukt, hergestellt über in-vitro- Transkription (IVT), exprimiert ein Luciferase-Protein, das ursprünglich aus dem Glühwürmchen isoliert wurde, Photinus pyralis. Glühwürmchen-Lucifrase (FLuc) wird häufig in der Säugetierzellkultur verwendet, um sowohl die Genexpression als auch die Zelllebensfähigkeit zu messen. In Gegenwart des Substrats Luciferin emittiert es Biolumineszenz.

Supplier: TriLink BioTechnologies

TriLink’s CleanCap® AG (3′ OMe) CleanScript™ IVT Kit provides essential components for mRNA synthesis by in vitro transcription (IVT) with co-transcriptional capping. The kit components are sufficient for 25×100 µl or 125×20 µl reactions following our recommended IVT protocol. It includes a cap analog, modified and unmodified nucleotides, IVT enzymes, a reaction buffer, and a control DNA template. Each kit is expected to yield 20-25 mg of capped mRNA.

Supplier: OZ BIOSCIENCES

AlumVax hydroxide is a wet gel (colloidal) of aluminium hydroxide 2%. It is the most common adjuvant used in approved prophylactic vaccines because of its excellent safety profile and ability to enhance the protective humoral immune response.

Supplier: Thermo Fisher Scientific

Imject™ Freund's Incomplete Adjuvant (FIA) is a popular non specific stimulator of the immune response for use in antibody production immunisation protocols.

Supplier: AAT Bioquest

This kit requires very little hands-on time, allowing for rapid protein expression screening in a variety of gel types using an imaging equipment with a standard FITC filter.

Supplier: OZ BIOSCIENCES

SqualVax is an oil-in-water emulsion made of squalene droplets in a continuous aqueous phase. It is an important advantage over alternative oils that have been used in emulsion adjuvants, like Freund’s adjuvant that contains mineral oil (paraffin oil) and has long term persistence in organisms.

Supplier: OZ BIOSCIENCES

Incomplete Freund’s Adjuvant (IFA) is a water-in-oil emulsion that is similar to the complete Freund’s Adjuvant (CFA) without addition of heat-killed mycobacteria (Mycobacterum butyricum).

Supplier: Thermo Fisher Scientific

The Thermo Scientific 1-Step Human High-Yield In Vitro Translation (IVT) Kits enable the expression of functional proteins with 10 to 100 times greater yield per mL than other mammalian IVT systems.

Supplier: Thermo Fisher Scientific

The 1-Step Human High-Yield In Vitro Translation (IVT) Kits enable the expression of functional proteins with 10 to 100 times greater yield per ml than other mammalian IVT systems.

Supplier: Thermo Fisher Scientific

The 1-Step Human Coupled IVT Kit – DNA is a mammalian in vitro translation (IVT) system based on HeLa cell lysates. The kit contains all of the cellular components required for protein synthesis, including ribosomes, initiation factors, elongation factors and tRNA. When supplemented with the included proprietary accessory proteins, reaction mix and a DNA template cloned into the Thermo Scientific pT7CFE1-based vector, this system can synthesise protein for up to 6 hours. The kit includes a pT7CFE-CHis vector for ease of use in downstream applications.

Supplier: Merck Millipore (Novagen)

The Overnight Express™ Autoinduction Systems enable regulated protein expression in E. coli, without monitoring the culture or adding inducer during cell growth.

Supplier: Thermo Fisher Scientific

Imject™ Freund's Complete Adjuvant (FCA) is a popular non specific stimulator of the immune response for use in antibody production immunisation protocols.

Supplier: OZ BIOSCIENCES

Complete Freund’s Adjuvant (CFA) is a water-in-oil emulsion containing 1 mg per ml heat-killed Mycobacterium tuberculosis.

Supplier: OZ BIOSCIENCES

AlumVax phosphate is a wet gel (colloidal) of aluminium phosphate 2%. Aluminium salts are the most common adjuvants used in approved prophylactic vaccines because of their excellent safety profile and ability to enhance the protective humoral immune response.

Supplier: Merck Millipore (Novagen)

The Overnight Express™ Autoinduction Systems enable regulated protein expression in E. coli, without monitoring the culture or adding inducer during cell growth.

Supplier: Thermo Fisher Scientific

The Pierce™ Rapid isotyping kit is a 5-minute lateral-flow assay with ELISA sensitivity for class and subclass determination of monoclonal antibodies.