Puffer
Pufferlösungen sind pH-beständig und schützen Proben bei Zugabe von Säuren oder Laugen. Sie sind als Pulver und Flüssigkeiten erhältlich und ermöglichen die effiziente Verfolgung von Proteinen während der elektrophoretischen Isolierung und Trennung. Salzhaltige Pufferlösungen eignen sich zudem zur Kalibrierung und Standardisierung von pH-Metern für präzise Messwerte. Blockierende Substanzen verhindern die Bindung von Antikörpern an freie Membranstellen, wodurch ein störender Hintergrund entstehen würde. Die Pufferlösungen sind in verschiedenen Konzentrationen, sowohl flüssig als auch dehydriert, erhältlich und decken somit jeden Bedarf ab.
MMP-3 fluorescent assay buffer
Supplier: Enzo Life Sciences
MMP-3 Fluorescent assay buffer
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G-Biosciences 10X Concentrated TE Buffers (Tris-EDTA Buffer)
Supplier: G-Biosciences
The tris buffering agent and EDTA metal chelating properties help protect DNA and RNA.
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Buffer solution pH 4, citrate buffer
Supplier: Thermo Fisher Scientific
pH Buffer solution, Citrat, pH-Wert: 4,00, ±0,02, Farblos
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Buffer, EDTA buffer solution pH 8,0 (0,5 mol/l)
Supplier: PanReac AppliChem
Cloning Buffer, EDTA buffer solution pH 8,0 (0,5 mol/l)
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Wash buffer concentrate
Supplier: Enzo Life Sciences
Liquid. TRIS buffered saline containing detergents.
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TBE-Puffer-Lösung 10fach konzentriert (TRIS-Borat-EDTA-Puffer)
Supplier: Rockland Immunochemicals
Laufpuffer, TBE-Puffer-Lösung 10fach konzentriert (TRIS-Borat-EDTA-Puffer)
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MMP-1 assay buffer
Supplier: Enzo Life Sciences
MMP-1 assay buffer
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TBE-Puffer-Lösung 5fach konzentriert (TRIS-Borat-EDTA-Puffer)
Supplier: Biotium
Laufpuffer, TBE-Puffer-Lösung 5fach konzentriert (TRIS-Borat-EDTA-Puffer)
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SDS-Page running gel buffers, 10X concentrate
Supplier: Rockland Immunochemicals
SDS-PAGE, sodium dodecyl sulfate polyacrylamide gel electrophoresis, describes a technique widely used in biochemistry to separate proteins according to their electrophoretic mobility (a function of the length of a polypeptide chain and its charge) and no other physical feature. SDS is an anionic detergent applied to protein sample to linearise proteins and to impart a negative charge to linearised proteins.
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Proteometer-CV mobile phase A salts
Supplier: NOVILYTIC
5 sealed salt packets specifically formulated for use with Proteometer-CV kit.
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Protein blocking buffers, Superior™
Supplier: G-Biosciences
Blocking buffer is an enhanced blocking agent available in multiple formats. Superior™ Blocking Buffer contains a proprietary antigenically non-determinant protein for blocking Western blot membranes, blocking and storage of ELISA plates, and blocking prior to immunohistochemistry staining.
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G-Biosciences Sodium Chloride-Sodium Citrate Buffer (20X)
Supplier: G-Biosciences
Saline-Sodium Citrate Hybridization Buffer (20X) controls stringency during the washing steps in Southern blotting, in situ hybridization, DNA microarray or Northern blotting.
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G-Biosciences Hybridization Buffer (Hybridization Neutralizing Solution)
Supplier: G-Biosciences
Ready-to-Use: Convenient, time-saving formulation ideal for consistent experimental reproducibility.
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G-Biosciences Hybridization Buffer (Hybridization Denaturing Solution)
Supplier: G-Biosciences
Ready-to-Use: Convenient, time-saving formulation ideal for consistent experimental reproducibility.
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Optimizer Buffer™ for optimal cross-linking and modification
Supplier: G-Biosciences
The conjugation and modification reactions used to cross-link proteins or couple labels to proteins, such as biotin, enzymes, and fluorescent dyes, require certain conditions, including pH and chemical composition for optimal conjugation. Many common buffers routinely used in laboratories have an inhibitory effect on conjugation reactions; for example, Tris buffers inhibit coupling to amines.
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Protein Transfer buffers
Supplier: G-Biosciences
Efficient™ Western Transfer Buffer (20X) achieves increased protein transfer efficiency. This ready-to-use transfer buffer is prepared for optimal conductivity and efficient protein transfer, without generating excessive heat or transfer distortion.
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