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Tests

An assay is an investigative procedure in laboratory medicine, pharmacology, environmental biology, and molecular biology for qualitatively assessing or quantitatively measuring the presence or functional activity of the analyte, or the entity being targeted by the assay. Generally, assays involve biological materials or phenomena which tend to be intrinsically more complex in composition or in behaviour than what can be handled by traditional chemical analysis and titration.

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Kolorimetrische DNMT-Tests für die Arzneimittelforschung

Kolorimetrische DNMT-Tests für die Arzneimittelforschung

Supplier: ENZO LIFE SCIENCES

Das kolorimetrische DNMT-Nachweiskit für die Arzneimittelforschung stellt ein einfaches Verfahren zum Nachweis der DNA-Methylierungsaktivität oder -hemmung dar.

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SARS-CoV-2 Antigen-Schnelltestkassette (Nasenabstrich)

SARS-CoV-2 Antigen-Schnelltestkassette (Nasenabstrich)

Supplier: SURESCREEN

Ein Schnelltest zum qualitativen Nachweis des SARS-CoV-2-Antigen in einem Nasenabstric.

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cGMP ELISA kit

Supplier: ENZO LIFE SCIENCES

Most sensitive (0,088pmol/m) colorimetric kit for extracellular cGMP quantification.

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Direct cGMP ELISA kit

Supplier: ENZO LIFE SCIENCES

Most sensitive (0,025pmol/m) colorimetric kit for intracellular cGMP quantification.

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8-iso-PGF2α ELISA kits

Supplier: ENZO LIFE SCIENCES

For the quantitative determination of 8-iso-PGF2α in culture supernatants, tissue, and urine from any species.  Cited sample type includes plasma.

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Oxytocin ELISA Kit

Oxytocin ELISA Kit

Supplier: Cayman Chemical

For the quantification of oxytocin in plasma.

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DuraClone Dry Unitized Antibody Panels For Flow Cytometry

DuraClone Dry Unitized Antibody Panels For Flow Cytometry

Supplier: Beckman Coulter

The dry DURAClone antibody panels are designed for clinical research studies. Every assay tube or well contains a layer with the dried-down antibody panel unitized for a single test.

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Direct cAMP ELISA kits

Supplier: ENZO LIFE SCIENCES

This direct cAMP ELISA kit is the most sensitive colorimetric kit for intracellular cAMP quantification. For the quantitative determination of cAMP in cell lysates and tissue from any species. Cited sample types include cell culture supernatant and tissue culture.

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Autophagie-Färbelösung, CYTO-ID®

Supplier: ENZO LIFE SCIENCES

CYTO-ID kann die Autophagozytose in lebenden Zellen und den autophagischen Fluss in lysosomal inhibierten lebenden Zellen mittels eines neuartigen Farbstoffs, der akkumulierte autophagische Vakuolen selektiv markiert, überwachen.

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LEADING LIGHT® Wnt Reporter Assay Starter Kit

Supplier: ENZO LIFE SCIENCES

The LEADING LIGHT® Wnt Reporter Assay Starter Kit is a cell-based luciferase activity test suitable for 96 well plate format.

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Corticosterone ELISA Kits

Corticosterone ELISA Kits

Supplier: Cayman Chemical

A competitive assay for the quantification of corticosterone in serum and rodent feces.

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Hypoxia/oxidative stress detection kit, ROS-ID®

Supplier: ENZO LIFE SCIENCES

The ROS-ID® Hypoxia/Oxidative Stress Detection Kit is a specific detection system designed for functional detection of hypoxia and oxidative stress levels in live cells (both suspension and adherent) using fluorescent microscopy or flow cytometry.

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Leukotriene E4 ELISA Kit

Leukotriene E4 ELISA Kit

Supplier: Cayman Chemical

For the quantification of LTE4 in urine.

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proBDNF Rapid ELISA Kit: Human, Mouse, Rat (1 Plate)

proBDNF Rapid ELISA Kit: Human, Mouse, Rat (1 Plate)

Supplier: Biosensis

The Biosensis proBDNF (Brain-derived neurotrophic factor, BDNF, Abrineurin) RapidTM enzyme-linked immunosorbent assay (ELISA) Kit is a sandwich ELISA that allows the specific, fast and reliable quantification of proBDNF in less than 4 hours in cell culture supernatants, human serum and EDTA-plasma only if used as directed. Please refer to the kit protocol for specific use instructions for each substrate application, in particular human blood samples.

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Rat IgA ELISA Kit

Supplier: BIOMOL RESEARCH LABORATORIES

This ELISA is for the detection of rat IgA in serum or plasma. Due to idiotypic and allotypic variations, this product may not accurately quantitate hybridomas, monoclonals or myelomas. This kit contains sufficient components to quantitate rat IgA in up to 40 samples, tested in duplicate.

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Cortisol ELISA Kit

Cortisol ELISA Kit

Supplier: Cayman Chemical

A competitive assay for the quantification of cortisol in urine, plasma, and other sample matrices.

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PROTEOSTAT® protein aggregation assay

PROTEOSTAT® protein aggregation assay

Supplier: ENZO LIFE SCIENCES

PROTEOSTAT® Protein aggregation assay provides a simple, homogenous assay format for monitoring peptide and protein aggregation in solution. This is useful for defining optimal storage formulations for proteins, for screening of compounds that promote or inhibit protein aggregation and potentially for the sensitive measurement of molecular chaperone activity. The assay can be employed to streamline protein processing and formulation optimization procedures.

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Protease Estimation Assay Kit, ProteSEEKER™

Protease Estimation Assay Kit, ProteSEEKER™

Supplier: G-Biosciences

ProteSEEKER™ identifies specific types of proteases with a panel of twelve protease inhibitors and a sensitive colorimetric protease screening assay. ProteSEEKER™ allows researchers to screen their protein samples and establish which specific class of proteases are present and therefore design a highly specific protease inhibitor cocktail using the minimal number of protease inhibitors. Alternatively, ProteSEEKER™ can be used to test existing protease inhibitor cocktails and identify their inadequacies and therefore supplement in additional protease inhibitors.

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proBDNF Rapid ELISA Kit: Human, Mouse, Rat (2 Plates)

proBDNF Rapid ELISA Kit: Human, Mouse, Rat (2 Plates)

Supplier: Biosensis

Biosensis proBDNF (Brain-derived neurotrophic factor, BDNF, Abrineurin) RapidTM enzyme-linked immunosorbent assay (ELISA) Kit is a sandwich ELISA that allows the specific, fast and reliable quantification of proBDNF in less than 4 hours in cell culture supernatants, human serum and EDTA-plasma only if used as directed. Please refer to the kit protocol for specific use instructions for each substrate application, in particular human blood samples.

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Calcium assay kits, FLIPR® Calcium 5 assay kit

Calcium assay kits, FLIPR® Calcium 5 assay kit

Supplier: Molecular Devices

The FLIPR® Calcium 5 Assay Kit provides a fast, simple, and reliable fluorescence-based assay for detecting changes in intracellular calcium.

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Cell viability assay kits, EarlyTox™ live/dead assay kits

Cell viability assay kits, EarlyTox™ live/dead assay kits

Supplier: Molecular Devices

EarlyTox™ Cell Viability Assay Kits are a family of fluorescence-based reagents for the assessment of cell viability, cell proliferation, and various apoptosis events using mammalian cells. Optimised for use with microplate readers, these assay kits employ a no-wash, homogeneous assay protocol that enables characterisation of a full concentration-response profile of test compounds.

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Auto-Ubiquitinylation Kit

Auto-Ubiquitinylation Kit

Supplier: ENZO LIFE SCIENCES

This E3 ligase auto-ubiquitinylation kit enables proteins to be tested for ubiquitin E3 ligase activity through assessment of their ability to under auto-ubiquitinylation. Utilizing the first three steps in the ubiquitin cascade the kit facilitates ubiquitinylation of known or putative E3 ligase enzymes followed by Western blot analysis using the highly sensitive reagents provided or using antibodies to the specific protein of interest (user supplied). A high integrity ubiquitin E3 ligase enzyme is also provided for use as a positive control.

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Cell viability assay kits, EarlyTox™ Caspase-3/7 and Caspase-3/7-D NucView™ 488 Assay Kits

Cell viability assay kits, EarlyTox™ Caspase-3/7 and Caspase-3/7-D NucView™ 488 Assay Kits

Supplier: Molecular Devices

EarlyTox™ Cell Viability Assay Kits are a family of fluorescence-based reagents for the assessment of cell viability, cell proliferation, and various apoptosis events using mammalian cells. Optimised for use with microplate readers, these assay kits employ a no-wash, homogeneous assay protocol that enables characterisation of a full concentration-response profile of test compounds.

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Mouse SERPINA3C ELISA Kit

Supplier: Aviva Systems Biology

Aviva Systems Biology Serpina3c ELISA Kit (Mouse) (OKWB00278) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Serpina3c has been pre-coated onto a 96-well plate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Serpina3c is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Serpina3c captured in the well.

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Human RHOT1 ELISA Kit

Supplier: Aviva Systems Biology

Aviva Systems Biology RHOT1 ELISA Kit (Human) (OKEH03665) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for RHOT1 has been pre-coated onto a 96-well plate (12×8 Well Strips) and blocked. Standards or test samples are added to the wells and removed. A biotinylated detector antibody specific for RHOT1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample RHOT1 captured in well.

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Calcium assay kits, FLIPR® Calcium 6 and 6-QF assay kits

Calcium assay kits, FLIPR® Calcium 6 and 6-QF assay kits

Supplier: Molecular Devices

The FLIPR® Calcium 6 and 6-QF Assay Kits provide a fast, simple, and reliable fluorescence based assay for detecting changes in intracellular calcium. The Calcium 6 Assay Kit significantly reduces fluorescence background with a one-step protocol. The Calcium 6-QF Assay Kit provides a quench-free option for sensitive targets or multiplexing applications.

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Cell viability assay kits, EarlyTox™ Caspase-3/7 R110 assay kit

Cell viability assay kits, EarlyTox™ Caspase-3/7 R110 assay kit

Supplier: Molecular Devices

EarlyTox™ Cell Viability Assay Kits are a family of fluorescence-based reagents for the assessment of cell viability, cell proliferation, and various apoptosis events using mammalian cells. Optimised for use with microplate readers, these assay kits employ a no-wash, homogeneous assay protocol that enables characterisation of a full concentration-response profile of test compounds.

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Fluorometric HTS assay kits, Caspase-3 and Caspase-8

Fluorometric HTS assay kits, Caspase-3 and Caspase-8

Supplier: Biotium

Caspase-3 and Caspase-8 Fluorometric HTS Assay Kits provide a homogenous assay system for fast and highly sensitive detection of Caspase-3 or Caspase-8 activity by fluorescence in enzymatic reaction or mammalian cells.

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proNGF Rapid™ ELISA Kit: Rat and Mouse

proNGF Rapid™ ELISA Kit: Rat and Mouse

Supplier: Biosensis

The Biosensis Mouse and Rat proNGF Rapid enzyme-linked immunosorbent assay (ELISA) Kit is a sandwich ELISA that allows the quantification of rodent full-length proNGF protein in less than 4 hours in cell culture supernatants, cell lysates and tissue homogenates only if used as directed. This ELISA Kit has also successfully been used on mouse urine samples (Ryu JC et al., 2018). Please refer to the kit protocol for specific use instructions for each substrate application.

This ELISA kit contains a recombinant mouse proNGF standard expressed in E.coli and consists of a pre-coated anti-proNGF capture antibody, a biotinylated anti-proNGF detection antibody and horseradish peroxidase (HRP)-conjugated streptavidin. The addition of a substrate (3,3',5,5'-tetramethylbenzidine, TMB) yields a colored reaction product which is directly proportional to the concentration of proNGF present in samples and protein standards. A proNGF positive control (QC sample) is provided to assure consistent assay performance. This ELISA kit detects rat proNGF due to high degree of homology (96%) with mouse proNGF based on amino acid sequence, and the ability of this kit in detecting proNGF in rat PC12 cell lysates and rat brain tissue homogenate. In the absence of a true rat proNGF standard, results may be expressed as 'mouse proNGF equivalents'. This ELISA kit shows only 20% reactivity with the human form of proNGF and is therefore not suitable to quantify human proNGF. No cross-reactivity was observed with mature mouse NGF and full-length proBDNF when tested in assay buffer.

The antibodies used in this ELISA kit bind epitopes within the pro-domain (capture) and mature domain (detection) of the protein, thus this ELISA assay does not detect the pro-domain peptide. This kit has not been tested for other applications. Sufficient amount of proNGF standard is supplied to allow for spike- and recovery experiments in order to validate this ELISA assay for other sample matrices if required.

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Maus B2M ELISA Kit

Supplier: Aviva Systems Biology

Das B2m ELISA-Kit (Maus) (OKEH00344) von Aviva Systems Biology basiert auf der standardmäßigen Sandwich-Enzym-Linked-Immunosorbent-Assay-Technologie. Ein für B2m spezifischer Antikörper wurde auf eine 96-Well-Platte (12×8-Well-Streifen) vorbeschichtet. Standards oder Testproben werden in die Vertiefungen gegeben, inkubiert und entnommen. Ein für B2m spezifischer biotinylierter Detektorantikörper wird hinzugefügt, inkubiert und anschließend gewaschen. Anschließend wird das Avidin-Peroxidase-Konjugat zugegeben, inkubiert und nicht gebundenes Konjugat abgewaschen. Durch die Zugabe von TMB-Substrat wird eine enzymatische Reaktion erzeugt, die durch HRP katalysiert wird und ein blaues Farbprodukt erzeugt, das sich nach Zugabe einer sauren Stopplösung in Gelb ändert. Die anhand der Absorption bei 450 nm abgelesene Dichte der Gelbfärbung ist quantitativ proportional zur Menge der in der Vertiefung eingefangenen Probe B2m.

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