Ihre Suche nach: Zellkultur-Ergänzungen und -Zusätze

Supplier: VWR Chemicals

Eigelb-Tellurit-Emulsion, VWR®

Supplier: VWR Chemicals

Beinhaltet eine Mischung von Protease-Inhibitoren: AEBSF, Aprotinin, E-64, Bestatin und Leupeptin. Jedes Gefäß kann in 1 ml deionisiertem Wasser zu einer 100X Lösung wiederhergestellt werden.

Supplier: VWR Chemicals

VWR® Trypsin-EDTA solutions are used in cell culture to remove adherent cells from a culture surface. Trypsin is a porcine pancreas-derived enzyme that is commonly used for the dissociation and disaggregation of anchorage-dependent mammalian cells and tissues. The concentration of trypsin necessary to separate cells from their substrate is dependent primarily on the cell type and the age of the culture. EDTA, a chelating agent, enhances enzymatic activity by removing calcium and magnesium ions. These ions obscure the peptide bonds on which trypsin acts as well as enhancing cell to cell adhesion.

Supplier: VWR Chemicals

Contains a mix of protease inhibitors: AEBSF, E-64, Bestatin, Pepstatin, Leupeptin and 1, 10-Phenanthroline. Each vial can be reconstituted in 1 ml dimethyl sulfoxide to form a 100X solution.

Supplier: VWR Chemicals

Eigelb-Emulsion, steril, VWR®

Supplier: VWR Chemicals

Der wasserlösliche Teil der autolysierten Hefe enthält einen Vitamin-B-Komplex.

Supplier: Biowest

L-Glutamin ist eine essentielle Aminosäure, die von praktisch allen in Kultur gezüchteten Säugetier- und Insektenzellen benötigt wird. Es ist ein entscheidender Bestandteil vieler Zellkulturmedien und dient als wichtige Energiequelle für Zellen in Kultur. L-Glutamin ist als Trockenpulver und als gefrorene Lösung sehr stabil. In flüssigen Medien oder Stammlösungen kann L-Glutamin jedoch relativ schnell abgebaut werden. L-Glutamin ist labiler in Zellkulturlösungen als andere Aminosäuren.

Supplier: HyClone products (Cytiva)

With HyrTryp™ cell dissociation reagent, an animal-derived component-free (ADCF) recombinant alternative to porcine trypsin, you can dissociate and harvest cells with confidence. Simply substitute it in your existing protocol.

Supplier: G-Biosciences

The PhosphataseArrest™ phosphatase inhibitor cocktails are ready to use 100X solutions that are simply added to either extraction buffers or samples.

Supplier: PROTIDE PHARMACEUTICALS

Serum replacement with similar properties to TCM™ but contains additional components for cell types that have required higher concentrations of serum (typically more than 10%). TM-235™ is defined and multi-purpose. Contains highly purified, heat-treated bovine serum albumin, heat-treated bovine transferrin and recombinant insulin.

Supplier: MP Biomedicals

M9 Minimal medium salts are suitable for the preparation of M9 Minimal Medium for use in propagation of E.coli and plasmid amplification.

Supplier: G-Biosciences

A broad range, 100X concentrated, ready-to-use protease inhibitor cocktail, Plant ProteaseArrest™ inhibits plant serine, cysteine and other plant specific proteases including aminopeptidases, aspartic and metalloproteases.

Supplier: G-Biosciences

A ready to use, 100X concentrated, broad range protease inhibitor cocktail that is fully compatible with 2D electrophoresis and subsequent mass spectrometry.

Supplier: G-Biosciences

Protease-PhosphataseArrest™ provides full protection of protein samples from proteases and phosphatases released during the preparation of cell and tissue lysates. Protease-PhosphataseArrest™ contains both irreversible and reversible protease inhibitors to inhibit serine, cysteine and other proteases along with five phosphatase inhibitors that target all the phosphatase categories: serine/threonine (Ser/Thr) specific, tyrosine specific and dual specificity phosphatases.

Supplier: HyClone products (Cytiva)

HyClone™ L-glutamine is used as a supplement in serum- and protein-free media. It supports the growth of cells that have high energy demands and synthesise large amounts of proteins and nucleic acids.

Supplier: G-Biosciences

Protease inhibitor set contains 12 ready to use individual protease inhibitors, each at a 100X concentration. Various concentrations and/or combinations of protease inhibitors may be used to inhibit a broad spectrum of protease activity. The Protease inhibitor set can be used to design specific protease inhibitor cocktails, supplement existing cocktails or to screen for specific protease classes.

Supplier: ENZO LIFE SCIENCES

Natural products are an unsurpassed source of chemical diversity and an ideal starting point for any screening program for pharmacologically active small molecules. Historically, natural products have been the most successful source of new drugs. The Screen-Well® natural product library offers over 500 isolated natural compounds of known structure and includes many compounds from ethnopharmacology. Compounds are provided in solution in DMSO. Individual compounds or subsets can be resupplied in gram quantities.

Supplier: Biowest

Concentrated solutions of amino acids, vitamins or non essential amino acids can be added to a basal medium to enrich it and the resulting medium used as a classical MEM or BME. The final concentration of the MEM or BME must be 1X.

Supplier: ENZO LIFE SCIENCES

The Screen-Well® epigenetics library contains 43 compounds with defined activity against enzymes which carry out epigenetic modification of lysine. It also includes DNA methylation inhibitors. Compounds are dissolved in DMSO at 10 mM and aliquoted into deep-well plates at 100 µl or 500 µl per well. The library is a useful tool for chemical genomics, assay development and other pharmacological applications. The library contains inhibitors of these important enzymes: HDACs, SIRTs, Lysine demethylases, HATs, Histone methyl transferases, DNA methyltransferases as well as SIRT activators. A variety of structurally and mechanistically different compound classes are included.

Supplier: Biowest

Versene can be used instead of trypsin. It is a chelating agent that disperses the cells by cutting the cytoplasmic bridges between them. Versene has the advantage of being able to withstand autoclave temperature, which is a guarantee of sterility. In addition, it is less aggressive cells than trypsin, which is useful for studies on cell growth.

Supplier: Biowest

Concentrated solutions of amino acids, vitamins or non essential amino acids can be added to a basal medium to enrich it and the resulting medium used as a classical MEM or BME. The final concentration of the MEM or BME must be 1X.

Supplier: PanReac AppliChem

Water-soluble part of autolysed yeast. Rich of ammonia and vitamins, especially of water-soluble vitamin B-complexes; suitable for addition to media.

Supplier: G-Biosciences

ProteCEASE™ is a dry format version of ProteaseArrest™ for large scale preparative applications and for those users who prefer reconstitution prior to use. ProteCEASE™ is a superior general protease inhibitor cocktail that is suitable for purification from mammalian, plant, bacteria and yeast samples. The cocktail contains both irreversible and reversible protease inhibitors to inhibit serine, cysteine and other proteases. EDTA is an optional component for inhibiting metalloproteases.

Supplier: PROTIDE PHARMACEUTICALS

A unique cryopreservation solution that does not contain DMSO, foetal bovine serum, or animal protein. pZerve™ is ready to use and does not require any dilution or further processing.

Supplier: HyClone products (Cytiva)

Supplements cell culture with concentrated amino acids. Manufactured to meet cGMP manufacturing standards and QC specifications.

Supplier: PROTIDE PHARMACEUTICALS

Serum replacement used primarily for human cells and the production of cell secreted proteins. TCH™ is also effective in culturing other mammalian cells without the use of animal proteins. Contains only human proteins (i.e., human serum albumin, human transferrin, recombinant insulin), which are heat treated.

Supplier: PROTIDE PHARMACEUTICALS

Serum supplement for the culturing of human progenitor cells. Effective in studying cancer stem cells, iPSC, and mesenchymal stem cells.

Supplier: G-Biosciences

Recom ProteaseArrest™ is a broad range, bacterial, 100X concentrated, ready to use protease inhibitor cocktail. Recom ProteaseArrest™ offers greater protection for recombinant proteins expressed and purified from bacteria. Recom ProteaseArrest™ inhibits bacterial serine, cysteine, metallo- and other bacterial specific proteases, including aminopeptidases and aspartic proteases.

Supplier: ENZO LIFE SCIENCES

Protease inhibitor cocktail

Supplier: ENZO LIFE SCIENCES

Structurally and mechanistically different compounds for studying the roles of pro- and anti-autophagic molecules in cells and in vitro applications.