Ihre Suche nach: Cell Culture Media

Supplier: Biowest

Ham's F10 medium is intended to support the growth of Chinese Hamster Ovary cells under serum-free conditions and other mammalian cell types with serum supplementation. It is a popular medium for growth of fastidious cell lines.

Supplier: Biowest

The Leibovitz L-15 media were formulated to promote the cell growth in medium not balanced in CO₂. The formulations were developed with the sodium bicarbonate buffer. The Leibovitz L-15 media are buffered by a complement of salts, free base amino acids and galactose so they can be used under conditions of free gaseous exchange with the atmosphere. When properly supplemented, L-15 Medium supports established cell lines, such as HEp-2 and LLC-MK2, as well as primary explants of embryonic and adult human tissue. Many viruses also have been successfully cultivated in this medium.

Supplier: Biowest

McCoy’s media were originally formulated for growth and support of lymphocytes. This final modification produced a medium identical to RPMI 1629. McCoy's 5A media supports the indefinite proliferation of Walker 256 carcinoma cells. In addition, it is excellent for the propagation of leukocytes, biopsy tissues, a broad range of human and rat normal or transformed cell types, the most current primary and continuous cell lines.

Supplier: Biowest

CRML was originally formulated for promoting strain L (Earle) cells under serum-free conditions. These media are also known for its ability to sustain cloning of monkey kidney cells and for growth of many other mammalian cell lines when supplemented with horse or calf serum.

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The long term cultivation of cells requires the addition of serum. When properly supplemented, Medium 199 has broad species applicability, particularly for cultivation of non-transformed cells. It is widely used in virology, vaccine production and in vitro cultivation of primary explants of mouse pancreatic epithelial and rat lens tissues.

Supplier: Biowest

Dulbecco's Modified Eagle Medium (DMEM) is a modification of Basal Medium Eagle (BME) which contains approximately four times as much of the vitamins and amino acids, and two to four times as much glucose (depending on the version) as the original formulation.

Supplier: Biowest

Ham's F14 medium was developed from Ham's F12 medium, which was originally developed for the serum-free clonal growth of Chinese Hamster Ovary (CHO) cells, lung cells and mouse L-cells. Ham's F14 contains a double concentration of amino acids compared to the Ham's F12 medium. This product is also supplemented with calcium chloride and ascorbic acid.

Supplier: Biowest

Minimum Essential Medium (MEM) with Earle's Balanced Salts is a modification of Eagle's earlier medium, Basal Medium Eagle (BME), containing higher concentrations of the essential nutrients. These media have demonstrated the ability to support a variety of normal and transformed cells in culture and contain Earle's Balanced Salts, which make them suitable for use in atmospheres charged with CO₂ gas.

Supplier: Biowest

Ham's F12 was originally developed for the serum-free clonal growth of Chinese Hamster Ovary (CHO) cells, lung cells and mouse L-cells. It is frequently used with dialysed serum, hormones, selenium and other supplements for serum-free cultures. It is the medium of choice for supporting the growth of cells of rodent origin, particularly rabbit and rat and has proved to be an excellent cloning medium for the culture of myeloma and hybrid cells.

Supplier: Biowest

Basal Medium Eagle is principally used for monolayer diploid or primary cell cultures. It is the simplest of the basal media with all essential components for growth. BME ideally supports cell lines such as HeLa, L-cells and primary mammalian fibroblasts. BME has also successfully been used for the growth of human transformed cell lines (particularly WI-38 and MRC-5).

Supplier: Biowest

RPMI are general purpose enriched media with extensive applications for a broad spectrum of mammalian and hybridoma cells, including human myeloma, mouse hybridoma, human leukocytes, and B and T lymphocytes. It was originally formulated for suspension cultures and monolayer culture of human leukaemia cells.

Supplier: Biowest

Schneider’s Insect Medium was developed to support the growth of excised imaginal discs from the fruit fly, Drosophila melanogaster. When supplemented with 5 to 20% heat-inactivated foetal bovine serum Schneider's mediuam has been found to support the rapid growth of both primary and established cultures of cells derived from Drosophila spp. and several other dipterans.

Supplier: Biowest

Glasgow MEM (GMEM) was originally formulated for growing BHK 21 cells. The original formulation is an Eagle's Basal Medium modified to contain two times the normal concentration of amino acids and vitamins. It is intended for use with adherent cell lines.

Supplier: Biowest

In 1976, Guilbert and Iscove demonstrated that precursor cells of erythrocytes and macrophages could be cultured in a reduced-serum medium supplemented with albumin, transferrin, lecithin, and selenium. Iscove's medium is a modification of Dulbecco's Modified Eagle's Medium (DME) containing selenium, additional amino acids and vitamins, sodium pyruvate, HEPES buffer, and potassium nitrate instead of ferric nitrate. Further studies have demonstrated that Iscove's medium will support murine B lymphocytes, haemopoietic tissue from bone marrow, B-cells stimulated with lipopolysaccharide, T-lymphocytes, and a variety of hybrid cells.

Supplier: Biowest

Studies to determine the nutritional requirements of many cells have been in progress since Eagle's first reports. The major essential nutrients were identified, and work became focussed on the media requirements of individual cell types. Many media designed for these purposes are now available. Among the first of these media, developed initially to study hormonal requirements of cells in culture, was a mixture of DMEM and Ham's F12 medium, known as DMEM-Ham's F12.

Supplier: Biowest

Dulbeccos Modified Eagle Medium (DMEM) ist so formuliert, dass es das Wachstum einer breiten Palette von Säugetierzellen bewahrt und aufrechterhält. Ergänzt mit fötalem Kälberserum ermöglicht DMEM auch die Vermehrung embryonaler Stammzellen von Mäusen.