Ihre Suche nach: Extraktionspuffer

Supplier: OMEGA BIO-TEK

SP2-Puffer, Omega Bio-Tek

Supplier: VWR Chemicals

Detergenzienfreier Puffer für die Isolierung von Proteinen aus Gewebekulturzellen. Zelllyse und die anschließende Isolierung der rohen zytoplasmischen Fraktion sind in weniger als 30 Minuten abgeschlossen.

Supplier: Avantor

J.T.Baker® Cell Lysis Solution Biotech Reagent ist ein vorformuliertes und gebrauchsfertiges cGMP-Detergens, das für die Extraktion und Rückgewinnung von AAV-Partikeln aus Produzenten-Zelllinien (HEK293-Eltern- und Suspensionslinien, SF9) optimiert ist. J.T.Baker® Cell Lysis Solution ist eine proprietäre Mischung aus nichtionischen Tensiden, frei von Octylphenolethoxylat, die eine vergleichbare Leistung wie Detergens nach Industriestandard aufweist und gleichzeitig die mit REACH und der nachgelagerten Freigabe verbundenen Umweltbedenken berücksichtigt. Diese Formulierung wurde für Gentherapie-Arbeitsabläufe entwickelt und ist einfach zu verwenden und zu handhaben, da sie gebrauchsfertig ist, eine niedrige Viskosität aufweist, keinen Transport oder keine Lagerung bei niedrigen Temperaturen erfordert und in Größen erhältlich ist, die Prozesse von der Entwicklung bis hin zu großen kommerziellen Chargen unterstützen. Eine detaillierte Beschreibung finden Sie in der technischen Literatur.

Supplier: OMEGA BIO-TEK

The E.Z.N.A.® RNALock Stabilizer Reagent is an innovative reagent which rapidly lyses cells and tissues to inactivate RNases at room temperature. Simply submerge the samples, such as cell cultures, animal tissues, or white blood cells into the RNALock Stabilizer Reagent. The intact cellular RNA will be preserved even at elevated temperature. Samples can be stored in this reagent in excess of 12 months at −20 °C.

Supplier: G-Biosciences

Gram-negative lysis buffer is an extraction buffer for soluble proteins from Gram-negative bacteria. It is a proprietary improvement on the lysozyme based lysis in combination with various salts and agents, which allows extraction of soluble proteins. This buffer has been tested for use with several widely used bacteria including E.coli strains.

Supplier: Avantor

J.T. Baker® Cell Lysis Solution. Optimized multi-component, ready-to-use solution for extraction and recovery of AAV particles from HEK293 or SF9 cells. The product offers quantitative recovery through efficient cell lysis and protection of vector particles against shear stress. The solution is formulated with non-ionic detergents which are octyl phenol ethoxylate free.

Supplier: Tonbo Biosciences

RBC Lysis Buffer (10X) is a concentrated ammonium chloride-based lysing reagent.

Supplier: ENZO LIFE SCIENCES

The RIPA liquid cell lysis buffer composes of 50 mM TRIS-hydrogen chloride, pH 7,4, containing 150 mM sodium chloride, 1 mM EDTA, 1 mM EGTA, 1,2% Triton X-100, 0,5% sodium deoxycholate, and 0,1% SDS.

Supplier: ENZO LIFE SCIENCES

Cell lysis buffer 4, Enzo Life Sciences

Supplier: AGILENT

Progress from mammalian cells to qPCR or QRT-PCR experiments without nucleic acid purification.

Supplier: ENZO LIFE SCIENCES

Concentrated lysis buffer for preparation of cell and tissue extracts.

Supplier: G-Biosciences

These DNase- and RNase-free buffers and reagents are designed for high quality research.

Supplier: ENZO LIFE SCIENCES

RIPA cell lysis buffer 2, Enzo Life Sciences

Supplier: ENZO LIFE SCIENCES

Caspase cell lysis buffer, Enzo Life Sciences

Supplier: G-Biosciences

Yeast PE LB™ kit has been developed for extraction of biologically active, soluble proteins from yeast cells. Yeast PE LB™ is a proprietary improvement on the lyticase (Zymolyase®) based spheroplast preparation and extraction of soluble proteins from yeast cells. Yeast PE LB™ is based on organic buffering agents and utilises a mild non-ionic detergent, chelating agent, and a proprietary combination of various salts and agents to enhance extraction and stability of proteins.

Supplier: G-Biosciences

Tissue PE LB™ has been developed for extraction of total biologically active, soluble proteins from animal tissues. Tissue PE LB™ is based on an organic buffer and utilises a mild non-ionic detergent, chelating agent, and a proprietary combination of various salts and agents to enhance extraction and stability of proteins. Depending on the required downstream application, additional agents such as reducing agents and protease inhibitors may be added into Tissue PE LB™. Tissue PE LB™ has been tested for use with a wide variety of fresh and frozen animal tissues.

Supplier: Thermo Fisher Scientific

Phenol-chloroform, is phenol supplied premixed with chloroform:isoamyl alcohol (25:24:1) and accompanying bottle of buffer to equilibrate at either pH 6,7 or pH 8,0.

Supplier: Thermo Fisher Scientific

This kit is ideal for segregation and enrichment of proteins from five different cellular compartments.

Supplier: AAT BIOQUEST

Die aktuelle Nachfrage nach RT-PCR-Tests von SARS-CoV-2 hat zu einem weltweiten Mangel an RNA-Extraktionskits geführt.

Supplier: G-Biosciences

Mammalian Cell PE LB™ has been developed for extraction of total biologically active, soluble proteins from mammalian cultured cells. The Mammalian Cell PE LB™ is based on organic buffering agents and utilises a mild non-ionic detergent, chelating agent, and a proprietary combination of various salts and agents to enhance extraction and stability of proteins. Depending on the required downstream application, additional agents such as reducing agents, phosphatase and protease inhibitors may be added into Mammalian Cell PE LB™. Mammalian Cell PE LB™ has been tested on a wide variety of mammalian cells and can be used for both suspension and adherent cells.

Supplier: G-Biosciences

Bacterial PE LB™ kit has been developed for the extraction of biologically active soluble proteins, including recombinant proteins and inclusion bodies, from bacterial cells. Bacterial PE LB™ is a proprietary improvement on the lysozyme based lysis method, which allows extraction of soluble proteins and concurrent removal of nucleic acids (DNA and RNA) released during cell lysis. The Bacterial PE LB™ lysis system eliminates viscosity build-up, allowing effective clarification with lower centrifugal forces.

Supplier: Thermo Fisher Scientific

This extraction reagent is formulated to effectively lyse yeast and bacterial cells to extract and solubilise proteins for analysis.

Supplier: OMEGA BIO-TEK

XP2-Bindungspuffer, Omega Bio-Tek

Supplier: AAT BIOQUEST

All our ReadiUse™ reagents require minimal hands-on time.

Supplier: Thermo Fisher Scientific

The RIPA lysis and extraction buffer is a high-quality, ready-to-use and fully disclosed formulation of a popular cell lysis reagent for cultured mammalian cells.

Supplier: PanReac AppliChem

Cationic detergent cetyltrimethylammonium bromide (CTAB) is used to liberate and complex with total cellular nucleic acids.

Supplier: G-Biosciences

FOCUS™ protein solubilisation buffer is a dry, urea-based buffer for protein solubilisation. It is a pre-mixed formulation of urea, thiourea, CHAPS and non detergent sulphobetaine for maximum solubilising strength.

Supplier: OMEGA BIO-TEK

RBB Buffer, 4000 ml, Omega Bio-Tek

Supplier: Thermo Fisher Scientific

The I-PER Insect Cell Protein Extraction Reagent enables gentle and effective extraction of soluble protein from baculovirus-infected insect cells grown in suspension or monolayer culture. The baculovirus insect cell expression system is an efficient and popular system for production of recombinant (eukaryotic) proteins in cell culture. Proteins expressed in baculoviral systems can be used for structural analyses, biochemical assays and a variety of other applications. I-PER Reagent maintains functionality of extracted proteins and is directly compatible with downstream applications such as protein assays, Western blotting and 6xHis-tagged protein purification.

Supplier: Thermo Fisher Scientific

The IP lysis buffer is a mammalian whole cell lysis reagent based on a modified RIPA buffer formulation without SDS.