Extraktionspuffer
Extraktionspuffer werden gebrauchsfertig geliefert und sind wirksam bei der Isolierung von Gesamt-RNA und anderen Proteinen. Sie verbessern die Proteinausbeute und die Komplexität der Proben aus Zellen und Geweben. Optimierte Extraktionsreagenzien steigern die Proteingewinnung in nativen oder denaturierenden Puffern, die mit nachgeschalteten Analyseverfahren kompatibel sind. Die Zugabe einer geeigneten Pufferlösung zu einem Proteingemisch während des Extraktionsprozesses kann die Stabilität der Proteinmoleküle verbessern, da diese Moleküle verschiedenen Kräften ausgesetzt sind, um sie für Untersuchungen zu isolieren.
NP-40 lysis buffer (2X)
Supplier: Thermo Fisher Scientific
NP-40 lysis buffer (2X), Thermo Fisher Scientific
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Triton® X-100 lysis buffer with glycerol (2X), pH 7,4
Supplier: Thermo Fisher Scientific
Triton® X-100 lysis buffer with glycerol (2X), pH 7.4, Thermo Fisher Scientific
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PIPES lysis buffer with NP-40 (2X)
Supplier: Thermo Fisher Scientific
PIPES lysis buffer with NP-40 (2X), Thermo Fisher Scientific
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Succinate, 0,2 M buffer solution, pH 5,0
Supplier: Thermo Fisher Scientific
Succinate, 0.2M buffer soln., pH 5.0, Thermo Fisher Scientific
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MES lysis buffer with Triton® X-100
Supplier: Thermo Fisher Scientific
MES lysis buffer with Triton® X-100, Thermo Fisher Scientific
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Lysis buffers, red blood cells
Supplier: G-Biosciences
These DNase- and RNase-free buffers and reagents are designed for high quality research.
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ACK Lysing Buffer (1x), TheraPEAK™
Supplier: LONZA
Ammonium-Chloride-Potassium (ACK) Lysing Buffer is used to lyse red blood cells in preparations containing white blood cells.
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HEPES protein extraction buffer (5X)
Supplier: Thermo Fisher Scientific
Thermo Fisher Scientific
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G-Biosciences ACD (Acid Citrate Dextrose) Solutions for Anticoagulants
Supplier: G-Biosciences
ACD (Acid Citrate Dextrose) Solution A and ACD (Acid Citrate Dextrose) Solution B are anticoagulants for whole blood and also act as acidifying agents to platelet poor plasma.
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MNE buffer, pH 6,5
Supplier: Thermo Fisher Scientific
MNE buffer, pH 6.5, Thermo Fisher Scientific
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Protein extraction reagent, BugBuster® HT, Millipore®
Supplier: Merck Millipore (Novagen)
BugBuster® HT Protein Extraction Reagent combines BugBuster® Protein Extraction Reagent and Benzonase® Nuclease in one convenient reagent. BugBuster® HT eliminates common bioprocessing problems resulting from traditional cell lysis procedures. Soluble proteins are gently extracted from E. coli without exposure to heat or oxidative damage and viscosity is eliminated by nucleic acid digestion in a single step. The resulting protein extract can easily be fractionated by conventional purification techniques. BugBuster HT is ideally suited for application in high throughput protein purifications.
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NP-40 lysis buffer, low salt
Supplier: Thermo Fisher Scientific
NP-40 lysis buffer, low salt, Thermo Fisher Scientific
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GTE buffer
Supplier: Thermo Fisher Scientific
Thermo Fisher Scientific
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HEPES lysis buffer with NP-40 (2X)
Supplier: Thermo Fisher Scientific
HEPES lysis buffer with NP-40 (2X), Thermo Fisher Scientific
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Protein extraction reagent, BugBuster® 10X, Millipore®
Supplier: Merck Millipore (Novagen)
BugBuster® 10X is a concentrated formulation of the proprietary detergents employed in BugBuster® without the addition of salts or buffer components. Concentrated BugBuster® provides a flexible alternative to the ready-to-use standard 1X BugBuster®, allowing user-defined dilution and addition of buffer components. BugBuster® 10X has all of the bioprocessing benefits of standard BugBuster® plus the freedom to control pH, reagent concentration, and buffer additives necessary for maximum extraction and activity of your target protein.
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RIPA Lysis and Extraction Buffer
Supplier: G-Biosciences
A complete lysis buffer for the release of cytoplasmic, membrane and nuclear proteins from adherent and suspension cultured mammalian cells. The RIPA lysis buffer is fully compatible with many applications, including reporter assays, protein assays, immunoassays and other protein purification techniques.
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