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Extraktionspuffer

Extraktionspuffer

Extraktionspuffer werden gebrauchsfertig geliefert und sind wirksam bei der Isolierung von Gesamt-RNA und anderen Proteinen. Sie verbessern die Proteinausbeute und die Komplexität der Proben aus Zellen und Geweben. Optimierte Extraktionsreagenzien steigern die Proteingewinnung in nativen oder denaturierenden Puffern, die mit nachgeschalteten Analyseverfahren kompatibel sind. Die Zugabe einer geeigneten Pufferlösung zu einem Proteingemisch während des Extraktionsprozesses kann die Stabilität der Proteinmoleküle verbessern, da diese Moleküle verschiedenen Kräften ausgesetzt sind, um sie für Untersuchungen zu isolieren.

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DNA/RNA clean-up buffers

Supplier: OMEGA BIO-TEK

Binding buffer XP2, Omega Bio-Tek

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ReadiUse™ Mammalian Cell Lysis Buffer *5X*

ReadiUse™ Mammalian Cell Lysis Buffer *5X*

Supplier: AAT Bioquest

All our ReadiUse™ reagents require minimal hands-on time.

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RIPA lysis and extraction buffer, Pierce™

RIPA lysis and extraction buffer, Pierce™

Supplier: Thermo Fisher Scientific

The RIPA lysis and extraction buffer is a high-quality, ready-to-use and fully disclosed formulation of a popular cell lysis reagent for cultured mammalian cells.

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Lysis buffer, CTAB

Lysis buffer, CTAB

Supplier: PanReac AppliChem

Cationic detergent cetyltrimethylammonium bromide (CTAB) is used to liberate and complex with total cellular nucleic acids.

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RBB-Puffer

Supplier: OMEGA BIO-TEK

RBB Buffer, 4000 ml, Omega Bio-Tek

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I-PER™ Insect Cell Protein Extraction Reagents

I-PER™ Insect Cell Protein Extraction Reagents

Supplier: Thermo Fisher Scientific

The I-PER Insect Cell Protein Extraction Reagent enables gentle and effective extraction of soluble protein from baculovirus-infected insect cells grown in suspension or monolayer culture. The baculovirus insect cell expression system is an efficient and popular system for production of recombinant (eukaryotic) proteins in cell culture. Proteins expressed in baculoviral systems can be used for structural analyses, biochemical assays and a variety of other applications. I-PER Reagent maintains functionality of extracted proteins and is directly compatible with downstream applications such as protein assays, Western blotting and 6xHis-tagged protein purification.

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Luciferase cell lysis buffer (2X), Pierce™

Luciferase cell lysis buffer (2X), Pierce™

Supplier: Thermo Fisher Scientific

The Thermo Scientific Pierce luciferase cell lysis buffer effectively lyses adherent cells or pelleted suspension cells for analysis of promoter expression in luciferase assays.

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IP lysis buffer, Pierce™

IP lysis buffer, Pierce™

Supplier: Thermo Fisher Scientific

The IP lysis buffer is a mammalian whole cell lysis reagent based on a modified RIPA buffer formulation without SDS.

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Protein solubilisation buffer

Protein solubilisation buffer

Supplier: G-Biosciences

FOCUS™ protein solubilisation buffer is a dry, urea-based buffer for protein solubilisation. It is a pre-mixed formulation of urea, thiourea, CHAPS and non detergent sulphobetaine for maximum solubilising strength.

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ReadiUse™ Yeast Cell Lysis Buffer, 5X

ReadiUse™ Yeast Cell Lysis Buffer, 5X

Supplier: AAT Bioquest

ReadiUse™ Yeast Cell Lysis Buffer is a biochemical reagent designed for efficient yeast cell lysis and protein solubilisation in laboratory applications.

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ReadiUse™ Bacterial Cell Lysis Buffer *5X* (10 ml)

ReadiUse™ Bacterial Cell Lysis Buffer *5X* (10 ml)

Supplier: AAT Bioquest

Cell lysis refers to the breaking down of cells, and it is often used to analyze specific cellular compositions such as proteins, lipids, nucleic acids, reporter molecules, cell signal molecules and other small biomolecules.

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Subcellular protein fractionation kit for tissues

Subcellular protein fractionation kit for tissues

Supplier: Thermo Fisher Scientific

This kit is ideal for cell fractionation and enrichment of proteins from five different cellular compartments within two hours.

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Y-PER™ Yeast Protein Extraction Reagent

Y-PER™ Yeast Protein Extraction Reagent

Supplier: Thermo Fisher Scientific

This reagent uses a mild detergent lysis procedure to efficiently release functionally active solubilised proteins.

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Research buffers and reagents

Research buffers and reagents

Supplier: G-Biosciences

These DNase- and RNase-free buffers and reagents are designed for high quality research.

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Protein extraction kit, Insect PE LB™

Protein extraction kit, Insect PE LB™

Supplier: G-Biosciences

Insect PE LB™ has been developed for extraction of total biologically active, soluble proteins from adherent or suspension cultured insect cells, including Sf9 and Sf21. Insect PE LB™ utilises a mild non-ionic detergent and a proprietary combination of various salts and agents to enhance extraction and stability of proteins. The Insect PE LB™ is fully compatible with downstream processes, such as electrophoresis and chromatography. Depending on the required downstream application, additional agents such as reducing agents and protease inhibitors may be added into Insect PE LB™.

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Mammalian protein extract reagents, M-PER™

Mammalian protein extract reagents, M-PER™

Supplier: Thermo Fisher Scientific

The M-PER Mammalian Protein Extraction Reagent is designed to provide highly efficient total protein extraction from cultured mammalian cells.

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