Extraktionspuffer
Extraktionspuffer werden gebrauchsfertig geliefert und sind wirksam bei der Isolierung von Gesamt-RNA und anderen Proteinen. Sie verbessern die Proteinausbeute und die Komplexität der Proben aus Zellen und Geweben. Optimierte Extraktionsreagenzien steigern die Proteingewinnung in nativen oder denaturierenden Puffern, die mit nachgeschalteten Analyseverfahren kompatibel sind. Die Zugabe einer geeigneten Pufferlösung zu einem Proteingemisch während des Extraktionsprozesses kann die Stabilität der Proteinmoleküle verbessern, da diese Moleküle verschiedenen Kräften ausgesetzt sind, um sie für Untersuchungen zu isolieren.
Native lysis Buffer, Abcam
Supplier: Abcam
Abcam’s 1X Native lysis buffer is an efficient means of cell lysis and protein solubilization for both adherent and suspension cultured mammalian cells.
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RIPA cell lysis buffer 2
Supplier: Enzo Life Sciences
RIPA cell lysis buffer 2, Enzo Life Sciences
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1X Red Blood Cell Lysis Buffer, Abcam
Supplier: Abcam
Red Blood Cell (RBC) Lysis Buffer ab286858 provides a quick and efficient method of lysing red blood cells. It is provided in a 1x concentrated format, and is also available as ab204733 in a 10x format.
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Extraction reagents
Supplier: Enzo Life Sciences
Concentrated lysis buffer for preparation of cell and tissue extracts.
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Nuclear Extraction Kit, Abcam
Supplier: Abcam
This Nuclear Extraction Kit is formulated for the quick and simple isolation of nuclear and cytoplasmic fractions from cultured cells and tissue homogenates that can be used successfully in our transcription factor assay kits.
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Cell Lysis Buffer, Abcam
Supplier: Abcam
ab152163 is a 10X Cell Lysis Buffer used to lyse cells under non-denaturing conditions.
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Extraction Buffer 5X PTR, Abcam
Supplier: Abcam
Extraction buffer 5X PTR is formulated for preparing cell or tissue extracts for use in immunoassays, with added phosphatase inhibitors and protease inhibitor aprotinin. - Cited in over 20 publications - Phosphatase and protease inhibitors – contains inhibitors to preserve sample integrity - Easy to use – dilute to 1X with distilled water before use, or use with extraction enhancer buffer 50X for enhanced extraction - Compatible with a variety of sample types
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Caspase cell lysis buffer
Supplier: Enzo Life Sciences
Caspase cell lysis buffer, Enzo Life Sciences
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ReadiUse™ Viral-RNA-Lysepuffer
Supplier: AAT Bioquest
Die aktuelle Nachfrage nach RT-PCR-Tests von SARS-CoV-2 hat zu einem weltweiten Mangel an RNA-Extraktionskits geführt.
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Protein extraction kit, tissue PE LB™
Supplier: G-Biosciences
Tissue PE LB™ has been developed for extraction of total biologically active, soluble proteins from animal tissues. Tissue PE LB™ is based on an organic buffer and utilises a mild non-ionic detergent, chelating agent, and a proprietary combination of various salts and agents to enhance extraction and stability of proteins. Depending on the required downstream application, additional agents such as reducing agents and protease inhibitors may be added into Tissue PE LB™. Tissue PE LB™ has been tested for use with a wide variety of fresh and frozen animal tissues.
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Protein extraction kit, Bacterial PE LB™
Supplier: G-Biosciences
Bacterial PE LB™ kit has been developed for the extraction of biologically active soluble proteins, including recombinant proteins and inclusion bodies, from bacterial cells. Bacterial PE LB™ is a proprietary improvement on the lysozyme based lysis method, which allows extraction of soluble proteins and concurrent removal of nucleic acids (DNA and RNA) released during cell lysis. The Bacterial PE LB™ lysis system eliminates viscosity build-up, allowing effective clarification with lower centrifugal forces.
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Subcellular Protein Fractionation Kit for Cultured Cells
Supplier: Thermo Fisher Scientific
This kit is ideal for segregation and enrichment of proteins from five different cellular compartments.
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Protein extraction kit, mammalian cell PE LB™
Supplier: G-Biosciences
Mammalian Cell PE LB™ has been developed for extraction of total biologically active, soluble proteins from mammalian cultured cells. The Mammalian Cell PE LB™ is based on organic buffering agents and utilises a mild non-ionic detergent, chelating agent, and a proprietary combination of various salts and agents to enhance extraction and stability of proteins. Depending on the required downstream application, additional agents such as reducing agents, phosphatase and protease inhibitors may be added into Mammalian Cell PE LB™. Mammalian Cell PE LB™ has been tested on a wide variety of mammalian cells and can be used for both suspension and adherent cells.
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Pierce™ DNA Extraction Buffer, Phenol:Chloroform and Tris Buffer
Supplier: Thermo Fisher Scientific
Phenol-chloroform, is phenol supplied premixed with chloroform:isoamyl alcohol (25:24:1) and accompanying bottle of buffer to equilibrate at either pH 6,7 or pH 8,0.
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Protein extraction kit, Yeast PE LB™
Supplier: G-Biosciences
Yeast PE LB™ kit has been developed for extraction of biologically active, soluble proteins from yeast cells. Yeast PE LB™ is a proprietary improvement on the lyticase (Zymolyase®) based spheroplast preparation and extraction of soluble proteins from yeast cells. Yeast PE LB™ is based on organic buffering agents and utilises a mild non-ionic detergent, chelating agent, and a proprietary combination of various salts and agents to enhance extraction and stability of proteins.
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DNA/RNA clean-up buffers
Supplier: OMEGA BIO-TEK
CP-Puffer, Omega Bio-Tek
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#: 20864.320
Lieferant: VWR Chemicals
#: 83640.320
Lieferant: VWR Chemicals
#: 31770.294
Lieferant: VWR Chemicals
#: 1.00030.2500
Lieferant: Merck
#: 83645.320
Lieferant: VWR Chemicals