Ihre Suche nach: Vektoren, Plasmide und Bibliotheken

Bei der molekularen Klonierung sind Vektoren DNA-Moleküle, die zur künstlichen Übertragung von fremdem Genmaterial in eine andere Zelle eingesetzt werden. Die vier wichtigsten Arten von Vektoren sind Plasmide, virale Vektoren, Cosmide und künstliche Chromosomen, wobei die Plasmide am häufigsten verwendet werden. Vektoren können zur Klonierung eingesetzt werden, und einige Vektoren sind speziell auf diesen Verwendungszweck zugeschnitten, während andere für die Transkription und Proteinexpression vorgesehen sind. Einfachere Transkriptionsvektoren werden zur Amplifikation des eingefügten genetischen Materials verwendet.

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Gaussia Luc vector for luciferase assays, expression vectors, Pierce™

Supplier: Thermo Fisher Scientific

Gaussia Luc vectors contain a gene cloned from the copepod, Gaussia princeps. The gene encodes a naturally-secreted bioluminescent Gaussia luciferase (approx. 20 kDa), which enables measurement of the reporter activity in media (for real-time assays) and in cell lysates.

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Expression vectors, pGEX

Supplier: Cytiva

The pGEX vectors have an expanded multiple cloning site (MCS) that contains six restriction sites. The expanded MCS facilitates the unidirectional cloning of cDNA inserts obtained from libraries constructed using many available lambda vectors. pGEX-6P-1, pGEX-6P-2, and pGEX-6P-3 each encode the recognition sequence for site-specific cleavage by PreScission Protease between the GST domain and the multiple cloning site. pGEX-4T-1, pGEX-4T-2, and pGEX-4T-3 are derived from pGEX-2T and contain a Thrombin recognition site. pGEX-5X-1, pGEX-5X-2, and pGEX5X-3 are derivatives of pGEX-3X and possess a Factor Xa recognition site.

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Cell-free protein synthesis, rapid translation system (RTS) vectors

Supplier: biotechrabbit

Expression vectors for PCR-based generation of linear templates.

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Polyadenylic acid (poly-A)

Supplier: Merck

Polyadenylic acid (poly-A) is used to evaluate binding on cationic liposomes doped with non-ionic nucleolipids. Poly-A is used in small molecule mRNA targeted drug development to evaluate the binding of potential therapeutic agents such as the isoquinoline group of alkaloids.